A validated system for ligation-free USER™ -based assembly of expression vectors for mammalian cell engineering

Anne Mathilde Lund, Helene Faustrup Kildegaard, Bjarne Gram Hansen, Dorte Koefoed Holm, Mikael Rørdam Andersen, Uffe Hasbro Mortensen

Research output: Contribution to conferencePosterResearchpeer-review

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Abstract

The development in the field of mammalian cell factories require fast and high-throughput methods, this means a high need for simpler and more efficient cloning techniques. For optimization of protein expression by genetic engineering and for allowing metabolic engineering in mammalian cells, a new versatile expression vector system was developed. This vector system applies the ligation-free uracilexcision cloning technique to construct mammalian expression vectors of multiple parts and with maximum flexibility.
Original languageEnglish
Publication date2013
Publication statusPublished - 2013
Event23rd ESACT meeting - Lille, France
Duration: 23 Jun 201326 Jun 2013

Conference

Conference23rd ESACT meeting
CountryFrance
CityLille
Period23/06/201326/06/2013

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