A validated system for ligation-free USER™ -based assembly of expression vectors for mammalian cell engineering

Anne Mathilde Lund; Helene Faustrup Kildegaard; Bjarne Gram Hansen; Dorte Koefoed Holm; Mikael Rørdam Andersen; Uffe Hasbro Mortensen

A validated system for ligation-free USER™ -based assembly of expression vectors for mammalian cell engineering

Anne Mathilde Lund, Helene Faustrup Kildegaard, Bjarne Gram Hansen, Dorte Koefoed Holm, Mikael Rørdam Andersen, Uffe Hasbro Mortensen

Novo Nordisk Foundation Center for Biosustainability

Novozymes A/S

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Abstract

The development in the field of mammalian cell factories require fast and high-throughput methods, this means a high need for simpler and more efficient cloning techniques. For optimization of protein expression by genetic engineering and for allowing metabolic engineering in mammalian cells, a new versatile expression vector system was developed. This vector system applies the ligation-free uracilexcision cloning technique to construct mammalian expression vectors of multiple parts and with maximum flexibility.

Original language	English
Publication date	2013
Publication status	Published - 2013
Event	23rd ESACT meeting - Lille, France Duration: 23 Jun 2013 → 26 Jun 2013

Conference

Conference	23rd ESACT meeting
Country/Territory	France
City	Lille
Period	23/06/2013 → 26/06/2013

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Prod21382433941387Accepted author manuscript, 28.5 MB

http://www.lepublicsystemepco.com/events.php?IDManif=640&IDModule=71&IDRub=155

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title = "A validated system for ligation-free USER{\texttrademark} -based assembly of expression vectors for mammalian cell engineering",

abstract = "The development in the field of mammalian cell factories require fast and high-throughput methods, this means a high need for simpler and more efficient cloning techniques. For optimization of protein expression by genetic engineering and for allowing metabolic engineering in mammalian cells, a new versatile expression vector system was developed. This vector system applies the ligation-free uracilexcision cloning technique to construct mammalian expression vectors of multiple parts and with maximum flexibility.",

author = "Lund, {Anne Mathilde} and Kildegaard, {Helene Faustrup} and Hansen, {Bjarne Gram} and Holm, {Dorte Koefoed} and Andersen, {Mikael R{\o}rdam} and Mortensen, {Uffe Hasbro}",

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T1 - A validated system for ligation-free USER™ -based assembly of expression vectors for mammalian cell engineering

AU - Lund, Anne Mathilde

AU - Kildegaard, Helene Faustrup

AU - Hansen, Bjarne Gram

AU - Holm, Dorte Koefoed

AU - Andersen, Mikael Rørdam

AU - Mortensen, Uffe Hasbro

PY - 2013

Y1 - 2013

N2 - The development in the field of mammalian cell factories require fast and high-throughput methods, this means a high need for simpler and more efficient cloning techniques. For optimization of protein expression by genetic engineering and for allowing metabolic engineering in mammalian cells, a new versatile expression vector system was developed. This vector system applies the ligation-free uracilexcision cloning technique to construct mammalian expression vectors of multiple parts and with maximum flexibility.

AB - The development in the field of mammalian cell factories require fast and high-throughput methods, this means a high need for simpler and more efficient cloning techniques. For optimization of protein expression by genetic engineering and for allowing metabolic engineering in mammalian cells, a new versatile expression vector system was developed. This vector system applies the ligation-free uracilexcision cloning technique to construct mammalian expression vectors of multiple parts and with maximum flexibility.

M3 - Poster

T2 - 23rd ESACT meeting

Y2 - 23 June 2013 through 26 June 2013

ER -

A validated system for ligation-free USER™ -based assembly of expression vectors for mammalian cell engineering

Abstract

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