A type III polyketide synthase from Rhizobium etli condenses malonyl CoAs to a heptaketide pyrone with unusually high catalytic efficiency

Marimuthu Jeya, Tae-Su Kim, Manish Kumar Tiwari, Jinglin Li, Huimin Zhao, Jung-Kul Lee

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A novel type III polyketide synthase (RePKS) from Rhizobium etli produced a heptaketide pyrone using acetyl-CoA and six molecules of malonyl-CoA. Its catalytic efficiency (k(cat)/K-m = 5230 mM(-1) min(-1)) for malonyl CoA was found to be the highest ever reported. Molecular dynamics studies revealed the unique features of RePKS.
Original languageEnglish
JournalMolecular Biosystems
Volume8
Issue number12
Pages (from-to)3103-3106
Number of pages4
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • catalytic efficiency
  • enzyme activity
  • Gram-Negative Aerobic Rods and Cocci Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Rhizobiaceae [06509] Rhizobium etli species
  • acetyl-CoA 72-89-9
  • heptaketide pyrone
  • malonyl-CoA 524-14-1
  • type III polyketide synthase RePKS
  • 04500, Mathematical biology and statistical methods
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10515, Biophysics - Biocybernetics
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 31000, Physiology and biochemistry of bacteria
  • 31500, Genetics of bacteria and viruses
  • Biochemistry and Molecular Biophysics
  • Computational Biology
  • molecular dynamics simulation mathematical and computer techniques
  • AAM55027.1 GenBank, EMBL, DDJB nucleotide sequence
  • Enzymology
  • Models and Simulations
  • Acetyl Coenzyme A
  • Amino Acid Sequence
  • Binding Sites
  • Catalysis
  • Malonyl Coenzyme A
  • Molecular Dynamics Simulation
  • Polyketide Synthases
  • Protein Binding
  • Protein Structure, Secondary
  • Pyrones
  • Rhizobium etli
  • Sequence Alignment
  • Substrate Specificity
  • 524-14-1 Malonyl Coenzyme A
  • 72-89-9 Acetyl Coenzyme A
  • 79956-01-7 Polyketide Synthases
  • acetyl coenzyme A
  • malonyl coenzyme A
  • polyketide synthase
  • pyrone derivative
  • amino acid sequence
  • article
  • binding site
  • catalysis
  • chemistry
  • enzyme specificity
  • enzymology
  • metabolism
  • molecular dynamics
  • protein binding
  • protein secondary structure
  • sequence alignment
  • BIOCHEMISTRY
  • CHALCONE SYNTHASE
  • SUBSTRATE-SPECIFICITY
  • BIOSYNTHESIS
  • EXPRESSION

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