A tumorsphere model of glioblastoma multiforme with intratumoral heterogeneity for quantitative analysis of cellular migration and drug response

Johann Mar Gudbergsson*, Serhii Kostrikov, Kasper Bendix Johnsen, Frederikke Petrine Fliedner, Christian Brøgger Stolberg, Nanna Humle, Anders Elias Hansen, Bjarne Winther Kristensen, Gunna Christiansen, Andreas Kjær, Thomas Lars Andresen, Meg Duroux

*Corresponding author for this work

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Abstract

Glioblastoma multiforme (GBM) is the most common and malignant type of primary brain tumor and is characterized by its sudden onset and invasive growth into the brain parenchyma. The invasive tumor cells evade conventional treatments and are thought to be responsible for the ubiquitous tumor regrowth. Understanding the behavior of these invasive tumor cells and their response to therapeutic agents could help improve patient outcome. In this study, we present a GBM tumorsphere migration model with high biological complexity to study migrating GBM cells in a quantitative and qualitative manner. We demonstrated that the in vitro migration model could be used to investigate both inhibition and stimulation of cell migration with oxaliplatin and GBM-derived extracellular vesicles, respectively. The intercellular heterogeneity within the GBM tumorspheres was examined by immunofluorescent staining of nestin/vimentin and GFAP, which showed nestin and vimentin being highly expressed in the periphery of tumorspheres and GFAP mostly in cells in the tumorsphere core. We further showed that this phenotypic gradient was present in vivo after implanting dissociated GBM tumorspheres, with the cells migrating away from the tumor being nestin-positive and GFAP-negative. These results indicate that GBM tumorsphere migration models, such as the one presented here, could provide a more detailed insight into GBM cell biology and prove highly relevant as a pre-clinical platform for drug screening and assessing drug response in the treatment of GBM.
Original languageEnglish
JournalExperimental Cell Research
Volume379
Issue number1
Pages (from-to)73-82
ISSN0014-4827
DOIs
Publication statusPublished - 2019

Keywords

  • Glioblastoma
  • GBM
  • Migration
  • Invasion
  • Nestin
  • GFAP
  • Tumorsphere
  • Extracellular vesicles
  • Oxaliplatin

Cite this

Gudbergsson, Johann Mar ; Kostrikov, Serhii ; Johnsen, Kasper Bendix ; Fliedner, Frederikke Petrine ; Stolberg, Christian Brøgger ; Humle, Nanna ; Hansen, Anders Elias ; Kristensen, Bjarne Winther ; Christiansen, Gunna ; Kjær, Andreas ; Andresen, Thomas Lars ; Duroux, Meg. / A tumorsphere model of glioblastoma multiforme with intratumoral heterogeneity for quantitative analysis of cellular migration and drug response. In: Experimental Cell Research. 2019 ; Vol. 379, No. 1. pp. 73-82.
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abstract = "Glioblastoma multiforme (GBM) is the most common and malignant type of primary brain tumor and is characterized by its sudden onset and invasive growth into the brain parenchyma. The invasive tumor cells evade conventional treatments and are thought to be responsible for the ubiquitous tumor regrowth. Understanding the behavior of these invasive tumor cells and their response to therapeutic agents could help improve patient outcome. In this study, we present a GBM tumorsphere migration model with high biological complexity to study migrating GBM cells in a quantitative and qualitative manner. We demonstrated that the in vitro migration model could be used to investigate both inhibition and stimulation of cell migration with oxaliplatin and GBM-derived extracellular vesicles, respectively. The intercellular heterogeneity within the GBM tumorspheres was examined by immunofluorescent staining of nestin/vimentin and GFAP, which showed nestin and vimentin being highly expressed in the periphery of tumorspheres and GFAP mostly in cells in the tumorsphere core. We further showed that this phenotypic gradient was present in vivo after implanting dissociated GBM tumorspheres, with the cells migrating away from the tumor being nestin-positive and GFAP-negative. These results indicate that GBM tumorsphere migration models, such as the one presented here, could provide a more detailed insight into GBM cell biology and prove highly relevant as a pre-clinical platform for drug screening and assessing drug response in the treatment of GBM.",
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A tumorsphere model of glioblastoma multiforme with intratumoral heterogeneity for quantitative analysis of cellular migration and drug response. / Gudbergsson, Johann Mar; Kostrikov, Serhii; Johnsen, Kasper Bendix; Fliedner, Frederikke Petrine; Stolberg, Christian Brøgger; Humle, Nanna; Hansen, Anders Elias; Kristensen, Bjarne Winther; Christiansen, Gunna; Kjær, Andreas ; Andresen, Thomas Lars; Duroux, Meg.

In: Experimental Cell Research, Vol. 379, No. 1, 2019, p. 73-82.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - A tumorsphere model of glioblastoma multiforme with intratumoral heterogeneity for quantitative analysis of cellular migration and drug response

AU - Gudbergsson, Johann Mar

AU - Kostrikov, Serhii

AU - Johnsen, Kasper Bendix

AU - Fliedner, Frederikke Petrine

AU - Stolberg, Christian Brøgger

AU - Humle, Nanna

AU - Hansen, Anders Elias

AU - Kristensen, Bjarne Winther

AU - Christiansen, Gunna

AU - Kjær, Andreas

AU - Andresen, Thomas Lars

AU - Duroux, Meg

PY - 2019

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N2 - Glioblastoma multiforme (GBM) is the most common and malignant type of primary brain tumor and is characterized by its sudden onset and invasive growth into the brain parenchyma. The invasive tumor cells evade conventional treatments and are thought to be responsible for the ubiquitous tumor regrowth. Understanding the behavior of these invasive tumor cells and their response to therapeutic agents could help improve patient outcome. In this study, we present a GBM tumorsphere migration model with high biological complexity to study migrating GBM cells in a quantitative and qualitative manner. We demonstrated that the in vitro migration model could be used to investigate both inhibition and stimulation of cell migration with oxaliplatin and GBM-derived extracellular vesicles, respectively. The intercellular heterogeneity within the GBM tumorspheres was examined by immunofluorescent staining of nestin/vimentin and GFAP, which showed nestin and vimentin being highly expressed in the periphery of tumorspheres and GFAP mostly in cells in the tumorsphere core. We further showed that this phenotypic gradient was present in vivo after implanting dissociated GBM tumorspheres, with the cells migrating away from the tumor being nestin-positive and GFAP-negative. These results indicate that GBM tumorsphere migration models, such as the one presented here, could provide a more detailed insight into GBM cell biology and prove highly relevant as a pre-clinical platform for drug screening and assessing drug response in the treatment of GBM.

AB - Glioblastoma multiforme (GBM) is the most common and malignant type of primary brain tumor and is characterized by its sudden onset and invasive growth into the brain parenchyma. The invasive tumor cells evade conventional treatments and are thought to be responsible for the ubiquitous tumor regrowth. Understanding the behavior of these invasive tumor cells and their response to therapeutic agents could help improve patient outcome. In this study, we present a GBM tumorsphere migration model with high biological complexity to study migrating GBM cells in a quantitative and qualitative manner. We demonstrated that the in vitro migration model could be used to investigate both inhibition and stimulation of cell migration with oxaliplatin and GBM-derived extracellular vesicles, respectively. The intercellular heterogeneity within the GBM tumorspheres was examined by immunofluorescent staining of nestin/vimentin and GFAP, which showed nestin and vimentin being highly expressed in the periphery of tumorspheres and GFAP mostly in cells in the tumorsphere core. We further showed that this phenotypic gradient was present in vivo after implanting dissociated GBM tumorspheres, with the cells migrating away from the tumor being nestin-positive and GFAP-negative. These results indicate that GBM tumorsphere migration models, such as the one presented here, could provide a more detailed insight into GBM cell biology and prove highly relevant as a pre-clinical platform for drug screening and assessing drug response in the treatment of GBM.

KW - Glioblastoma

KW - GBM

KW - Migration

KW - Invasion

KW - Nestin

KW - GFAP

KW - Tumorsphere

KW - Extracellular vesicles

KW - Oxaliplatin

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DO - 10.1016/j.yexcr.2019.03.031

M3 - Journal article

VL - 379

SP - 73

EP - 82

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 1

ER -