TY - JOUR
T1 - A Systematic Study on the Physicochemical Interactions Between Polymeric Micelles and Mucin
T2 - Toward the Development of Optimal Drug Delivery Nanocarriers
AU - Tollemeto, Matteo
AU - Badillo-Ramírez, Isidro
AU - Thamdrup, Lasse Højlund Eklund
AU - Li, Yudong
AU - Ghavami, Mahdi
AU - Padial, Tania Patiño
AU - Christensen, Jørn B.
AU - van Hest, Jan
AU - Boisen, Anja
N1 - Publisher Copyright:
© 2024 The Authors. Advanced Materials Interfaces published by Wiley-VCH GmbH.
PY - 2024
Y1 - 2024
N2 - The optimal performance of drug delivery formulations, including polymeric nanoparticles, relies on particle distribution throughout the body and the interactions with biological barriers, particularly mucosal layers, which often limit their potential. A systematic and comprehensive study is presented through a multidisciplinary approach combining conventional and novel techniques for in vitro studies to understand the key molecular interactions between polymeric micelles and mucin. The results shows that polymeric micelles are integrates within the mucin layer, mirroring its viscoelastic properties, evidenced as a dissipation difference of 0.1 ± 0.44, measured by quartz crystal microbalance with dissipation. Surface-enhanced Raman scattering reveals predominant hydrogen bonding within the mucin's hydrophilic core, while the isothermal titration calorimetry method confirms multiple non-specific binding sites on the protein backbone. By performing the periodic acid-Schiff stain assay, a binding amount of 0.20 mg of mucin per milligram of nanoparticles is quantified. Furthermore, motility studies show the surface binding of mucin on the polymeric nanoparticles influencing their Brownian motion. This study sheds light toward the improvement for a better drug delivery formulation and fabrication of optimal nanoparticle colloidal systems, which can advance translational drug delivery technologies into clinical application while enriching the field of surface and colloidal chemistry.
AB - The optimal performance of drug delivery formulations, including polymeric nanoparticles, relies on particle distribution throughout the body and the interactions with biological barriers, particularly mucosal layers, which often limit their potential. A systematic and comprehensive study is presented through a multidisciplinary approach combining conventional and novel techniques for in vitro studies to understand the key molecular interactions between polymeric micelles and mucin. The results shows that polymeric micelles are integrates within the mucin layer, mirroring its viscoelastic properties, evidenced as a dissipation difference of 0.1 ± 0.44, measured by quartz crystal microbalance with dissipation. Surface-enhanced Raman scattering reveals predominant hydrogen bonding within the mucin's hydrophilic core, while the isothermal titration calorimetry method confirms multiple non-specific binding sites on the protein backbone. By performing the periodic acid-Schiff stain assay, a binding amount of 0.20 mg of mucin per milligram of nanoparticles is quantified. Furthermore, motility studies show the surface binding of mucin on the polymeric nanoparticles influencing their Brownian motion. This study sheds light toward the improvement for a better drug delivery formulation and fabrication of optimal nanoparticle colloidal systems, which can advance translational drug delivery technologies into clinical application while enriching the field of surface and colloidal chemistry.
KW - Mucoadhesion
KW - Mucosal barriers
KW - Nanomedicine
KW - Oral delivery
KW - Polymeric micelles
U2 - 10.1002/admi.202400107
DO - 10.1002/admi.202400107
M3 - Journal article
AN - SCOPUS:85192164720
SN - 2196-7350
VL - 11
JO - Advanced Materials Interfaces
JF - Advanced Materials Interfaces
IS - 19
M1 - 2400107
ER -