A system for improved production titers in fermentations

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The invention provides a genetically modified micro-organism for intracellular biosynthesis of a cellular metabolite, comprising a synthetic error correction system having a penalty gene, whose expression leads to arrested growth or cell death (e.g. a toxin gene) in combination with a survival gene, whose expression provides an antidote that restores cell viability and normal growth (e.g. a cognate antitoxin gene). Alternatively, the system has a survival gene, alone, whose expression is essential for growth (i.e. essential gene). The synthetic error correction system further comprises a biosensor, whose function is to induce expression of the survival gene which leads to cell growth, only, when the cell produces a pre-defined level of a given metabolite. The invention further encompasses: a method for producing the genetically modified micro-organism; a method for producing a cellular metabolite with the genetically modified micro-organism; and use of the genetically modified micro-organism for producing a cellular metabolite.

Original languageEnglish
IPCC12P 1/ 04 A I
Patent numberWO2017055360
Filing date06/04/2017
CountryInternational Bureau of the World Intellectual Property Organization (WIPO)
Priority date28/09/2015
Priority numberEP20150187150
Publication statusPublished - 6 Apr 2017

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