A synthetic glycan microarray enables epitope mapping of plant cell wall glycan-directed antibodies

Colin Ruprecht, Max P Bartetzko, Deborah Senf, Pietro Dallabernardina, Irene Boos, Mathias Christian Franch Andersen, Toshihisa Kotake, J. Paul Knox, Michael G. Hahn, Mads Hartvig Clausen, Fabian Pfrengle

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Abstract

In the last three decades, more than 200 monoclonal antibodies have been raised against most classes of plant cell wall polysaccharides by different laboratories world-wide. These antibodies are widely used to identify differences in plant cell wall components in mutants, organ and tissue types, and developmental stages. Despite their importance and broad use, the precise binding epitope for only a few of these antibodies has been determined. Here, we use a plant glycan microarray equipped with 88 synthetic oligosaccharides to comprehensively map the epitopes of plant cell wall glycan-directed antibodies. Our results reveal the binding epitopes for 78 arabinogalactan-, rhamnogalacturonan-, xylan-, and xyloglucan-directed antibodies. We demonstrate that, with knowledge of the exact epitopes recognized by individual antibodies, specific glycosyl hydrolases can be implemented into immunological cell wall analyses, providing a framework to obtain structural information on plant cell wall glycans with unprecedented molecular precision.
Original languageEnglish
JournalPlant Physiology
Volume175
Issue number3
Pages (from-to)1094-1104
ISSN0032-0889
DOIs
Publication statusPublished - 2017

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