A sustainable approach for the green synthesis of silver nanoparticles from Solibacillus isronensis sp. And their application in biofilm inhibition

Priyanka Singh, Santosh Pandit, V. R.S.S. Mokkapati, Jørgen Garnæs, Ivan Mijakovic*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

The use of bacteria as nanofactories for the green synthesis of nanoparticles is considered a sustainable approach, owing to the stability, biocompatibility, high yields and facile synthesis of nanoparticles. The green synthesis provides the coating or capping of biomolecules on nanoparticles surface, which confer their biological activity. In this study, we report green synthesis of silver nanoparticles (AgNPs) by an environmental isolate; named as AgNPs1, which showed 100% 16S rRNA sequence similarity with Solibacillus isronensis. UV/visible analysis (UV/Vis), transmission electron microscopy (TEM), atomic force microscopy (AFM), dynamic light scattering (DLS), and Fouriertransform infrared spectroscopy (FTIR) were used to characterize the synthesized nanoparticles. The stable nature of nanoparticles was studied by thermogravimetric analysis (TGA) and inductively coupled plasma mass spectrometry (ICP-MS). Further, these nanoparticles were tested for biofilm inhibition against Escherichia coli and Pseudomonas aeruginosa. The AgNPs showed minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 3.12 μg/mL and 6.25 μg/mL for E. coli, and 1.56 μg/mL and 3.12 μg/mL for P. aeruginosa, respectively.

Original languageEnglish
Article number2783
JournalMolecules
Volume25
Issue number12
ISSN1420-3049
DOIs
Publication statusPublished - Jun 2020

Keywords

  • E. coli
  • P. aeruginosa
  • Silver nanoparticles (AgNPs), biofilm inhibition
  • Solibacillus isronensis

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