A Surface Plasmon Resonance Immunosensor for Detection of urediniospores from Puccinia striiformis f. sp. tritici

Peter Skottrup, Stephen Hearty, Hanne Frøkiær, Paul Leonard, Jørn Hejgaard, Richard O’Kennedy, Mogens Nicolaisen, Annemarie Fejer Justesen

    Research output: Contribution to conferencePosterResearchpeer-review

    Abstract

    This study describes a generic biosensing principle for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a mouse monoclonal antibody (mAb) and a SPR sensor for label-free detection of the model organism Puccinia striiformis f.sp. tritici (Pst). We developed mAbs towards intact whole spores and used a subtractive inhibition format for detection of spores in solution. Assay conditions was optimised and a detection limit of 3.9 x 103 spores/ml was achieved. We further examined spiked Pst samples in a background of a related spore and found that Pst quantification was possible in this complex matrix. To the best of our knowledge, this study simultaneously represents the first use of SPR technology for fungal spore detection and the first report of a successful biosensor-based detection strategy for Pst
    Original languageEnglish
    Publication date2006
    Publication statusPublished - 2006
    Event8th European Conference on Optical Chemical and Biosensors - Tübingen, Germany
    Duration: 1 Jan 2006 → …

    Conference

    Conference8th European Conference on Optical Chemical and Biosensors
    CityTübingen, Germany
    Period01/01/2006 → …

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