A Surface Plasmon Resonance Immunosensor for Detection of urediniospores from Puccinia striiformis f. sp. tritici

Peter Skottrup; Stephen Hearty; Hanne Frøkiær; Paul Leonard; Jørn Hejgaard; Richard O’Kennedy; Mogens Nicolaisen; Annemarie Fejer Justesen

A Surface Plasmon Resonance Immunosensor for Detection of urediniospores from Puccinia striiformis f. sp. tritici

Peter Skottrup, Stephen Hearty, Hanne Frøkiær, Paul Leonard, Jørn Hejgaard, Richard O’Kennedy, Mogens Nicolaisen, Annemarie Fejer Justesen

Research output: Contribution to conference › Poster › Research › peer-review

Abstract

This study describes a generic biosensing principle for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a mouse monoclonal antibody (mAb) and a SPR sensor for label-free detection of the model organism Puccinia striiformis f.sp. tritici (Pst). We developed mAbs towards intact whole spores and used a subtractive inhibition format for detection of spores in solution. Assay conditions was optimised and a detection limit of 3.9 x 103 spores/ml was achieved. We further examined spiked Pst samples in a background of a related spore and found that Pst quantification was possible in this complex matrix. To the best of our knowledge, this study simultaneously represents the first use of SPR technology for fungal spore detection and the first report of a successful biosensor-based detection strategy for Pst

Original language	English
Publication date	2006
Publication status	Published - 2006
Event	8th European Conference on Optical Chemical and Biosensors - Tübingen, Germany Duration: 2 Apr 2006 → 4 Apr 2006 Conference number: 8

Conference

Conference	8th European Conference on Optical Chemical and Biosensors
Number	8
Country/Territory	Germany
City	Tübingen
Period	02/04/2006 → 04/04/2006

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@conference{951dc7d8c8d44c4baf430669fa3dc2a9,

title = "A Surface Plasmon Resonance Immunosensor for Detection of urediniospores from Puccinia striiformis f. sp. tritici",

abstract = "This study describes a generic biosensing principle for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a mouse monoclonal antibody (mAb) and a SPR sensor for label-free detection of the model organism Puccinia striiformis f.sp. tritici (Pst). We developed mAbs towards intact whole spores and used a subtractive inhibition format for detection of spores in solution. Assay conditions was optimised and a detection limit of 3.9 x 103 spores/ml was achieved. We further examined spiked Pst samples in a background of a related spore and found that Pst quantification was possible in this complex matrix. To the best of our knowledge, this study simultaneously represents the first use of SPR technology for fungal spore detection and the first report of a successful biosensor-based detection strategy for Pst",

author = "Peter Skottrup and Stephen Hearty and Hanne Fr{\o}ki{\ae}r and Paul Leonard and J{\o}rn Hejgaard and Richard O{\textquoteright}Kennedy and Mogens Nicolaisen and Justesen, {Annemarie Fejer}",

year = "2006",

language = "English",

note = "8th European Conference on Optical Chemical and Biosensors ; Conference date: 02-04-2006 Through 04-04-2006",

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A Surface Plasmon Resonance Immunosensor for Detection of urediniospores from Puccinia striiformis f. sp. tritici. / Skottrup, Peter; Hearty, Stephen; Frøkiær, Hanne et al.
2006. Poster session presented at 8th European Conference on Optical Chemical and Biosensors, Tübingen, Baden-Württemberg, Germany.

Research output: Contribution to conference › Poster › Research › peer-review

TY - CONF

T1 - A Surface Plasmon Resonance Immunosensor for Detection of urediniospores from Puccinia striiformis f. sp. tritici

AU - Skottrup, Peter

AU - Hearty, Stephen

AU - Frøkiær, Hanne

AU - Leonard, Paul

AU - Hejgaard, Jørn

AU - O’Kennedy, Richard

AU - Nicolaisen, Mogens

AU - Justesen, Annemarie Fejer

N1 - Conference code: 8

PY - 2006

Y1 - 2006

N2 - This study describes a generic biosensing principle for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a mouse monoclonal antibody (mAb) and a SPR sensor for label-free detection of the model organism Puccinia striiformis f.sp. tritici (Pst). We developed mAbs towards intact whole spores and used a subtractive inhibition format for detection of spores in solution. Assay conditions was optimised and a detection limit of 3.9 x 103 spores/ml was achieved. We further examined spiked Pst samples in a background of a related spore and found that Pst quantification was possible in this complex matrix. To the best of our knowledge, this study simultaneously represents the first use of SPR technology for fungal spore detection and the first report of a successful biosensor-based detection strategy for Pst

AB - This study describes a generic biosensing principle for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a mouse monoclonal antibody (mAb) and a SPR sensor for label-free detection of the model organism Puccinia striiformis f.sp. tritici (Pst). We developed mAbs towards intact whole spores and used a subtractive inhibition format for detection of spores in solution. Assay conditions was optimised and a detection limit of 3.9 x 103 spores/ml was achieved. We further examined spiked Pst samples in a background of a related spore and found that Pst quantification was possible in this complex matrix. To the best of our knowledge, this study simultaneously represents the first use of SPR technology for fungal spore detection and the first report of a successful biosensor-based detection strategy for Pst

M3 - Poster

T2 - 8th European Conference on Optical Chemical and Biosensors

Y2 - 2 April 2006 through 4 April 2006

ER -