TY - JOUR
T1 - A structural analysis of M protein in coronavirus assembly and morphology
AU - Neuman, Benjamin W.
AU - Kiss, Gabriella
AU - Kunding, Andreas Hjarne
AU - Bhella, David
AU - Baksh, M. Fazil
AU - Connelly, Stephen
AU - Droese, Ben
AU - Klaus, Joseph P.
AU - Makino, Shinji
AU - Sawicki, Stanley G.
AU - Siddell, Stuart G.
AU - Stamou, Dimitrios G.
AU - Wilson, Ian A.
AU - Kuhn, Peter
AU - Buchmeier, Michael J.
PY - 2011
Y1 - 2011
N2 - The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into
workshops where virus and host factors come together to make new virus particles. We investigated
how M structure and organization is related to virus shape and size using cryo-electron microscopy,
tomography and statistical analysis. We present evidence that suggests M can adopt two conformations
and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with
rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M
protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles
and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation,
presumably through interactions with M. These findings provide insight into how M protein functions
to promote virus assembly.
Keyword: Cryo-electron microscopy,Viral matrix protein,Coronavirus,Pleomorphic virus structure,Cryo-electron tomography
AB - The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into
workshops where virus and host factors come together to make new virus particles. We investigated
how M structure and organization is related to virus shape and size using cryo-electron microscopy,
tomography and statistical analysis. We present evidence that suggests M can adopt two conformations
and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with
rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M
protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles
and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation,
presumably through interactions with M. These findings provide insight into how M protein functions
to promote virus assembly.
Keyword: Cryo-electron microscopy,Viral matrix protein,Coronavirus,Pleomorphic virus structure,Cryo-electron tomography
U2 - 10.1016/j.jsb.2010.11.021
DO - 10.1016/j.jsb.2010.11.021
M3 - Journal article
SN - 1047-8477
VL - 174
SP - 11
EP - 22
JO - Journal of Structural Biology
JF - Journal of Structural Biology
ER -