A stable-isotope mass spectrometry-based metabolic footprinting approach to analyze exudates from phytoplankton

Ralf J. M. Weber, Erik Selander, Ulf Sommer, Mark R. Viant

Research output: Contribution to journalJournal articleResearchpeer-review

288 Downloads (Pure)

Abstract

Phytoplankton exudates play an important role in pelagic ecology and biogeochemical cycles of elements. Exuded compounds fuel the microbial food web and often encompass bioactive secondary metabolites like sex pheromones, allelochemicals, antibiotics, or feeding attractants that mediate biological interactions. Despite this importance, little is known about the bioactive compounds present in phytoplankton exudates. We report a stable-isotope metabolic footprinting method to characterise exudates from aquatic autotrophs. Exudates from 13C-enriched alga were concentrated by solid phase extraction and analysed by high-resolution Fourier transform ion cyclotron resonance mass spectrometry. We used the harmful algal bloom forming dinoflagellate Alexandrium tamarense to prove the method. An algorithm was developed to automatically pinpoint just those metabolites with highly 13C-enriched isotope signatures, allowing us to discover algal exudates from the complex seawater background. The stable-isotope pattern (SIP) of the detected metabolites then allowed for more accurate assignment to an empirical formula, a critical first step in their identification. This automated workflow provides an effective way to explore the chemical nature of the solutes exuded from phytoplankton cells and will facilitate the discovery of novel dissolved bioactive compounds.
Original languageEnglish
JournalMarine Drugs
Volume11
Pages (from-to)4158-4175
ISSN1660-3397
DOIs
Publication statusPublished - 2013

Keywords

  • Biology and Life Sciences
  • Health Sciences

Fingerprint Dive into the research topics of 'A stable-isotope mass spectrometry-based metabolic footprinting approach to analyze exudates from phytoplankton'. Together they form a unique fingerprint.

Cite this