A small cryptic plasmid from Ruminobacter amylophilus NIAH-3 possesses functional mobilization properties

K. Ogata, T. Sekizaki, Rustam Aminov, K. Tajima, M. Nakamura, T. Nagamine, H. Matsui, Y. Benno

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Abstract

The complete nucleotide sequence of a small cryptic plasmid designated pRAO1, from the Gram-negative ruminal bacterium Ruminobacter amylophilus NIAH-3, was determined. The plasmid is a circular DNA molecule, 2140 bp in size, with a GC content of 40%. Computer-assisted analysis identified three open reading frames (ORFs), one of which, ORF3 (347 amino acids), displayed a high degree of amino acid identity with the Mob proteins involved in conjugative mobilization and interplasmid recombination of plasmids from Gram-positive bacteria. We proved the mobilization properties of pRAO1 in the Escherichia coli system using the coresident IncW broad-host-range conjugative plasmid R388. These data demonstrated, for the first time, the mobilization properties of small cryptic plasmids from Gram-negative inhabitants of the rumen. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Original languageEnglish
JournalFEMS Microbiology Letters
Volume181
Issue number1
Pages (from-to)41-48
Number of pages8
ISSN0378-1097
DOIs
Publication statusPublished - 1999
Externally publishedYes

Keywords

  • Amino Acid Sequence
  • Animals
  • Bacterial Proteins
  • Base Sequence
  • Conjugation, Genetic
  • DNA, Bacterial
  • DNA, Circular
  • DNA-Binding Proteins
  • Escherichia coli
  • Gammaproteobacteria
  • Gram-Negative Bacteria
  • Molecular Sequence Data
  • Phylogeny
  • Plasmids
  • Rumen
  • Sequence Analysis, DNA
  • Mob protein, Bacteria
  • article
  • DNA sequence
  • mobilization
  • nonhuman
  • nucleotide sequence
  • open reading frame
  • phylogeny
  • plasmid
  • priority journal
  • rumen microorganism
  • sequence analysis
  • Bacteria (microorganisms)
  • Negibacteria
  • Posibacteria
  • Ruminobacter amylophilus
  • Mobilizable cryptic plasmid
  • MICROBIOLOGY
  • COMPLETE NUCLEOTIDE-SEQUENCE
  • BACTEROIDES-AMYLOPHILUS
  • GENUS BUTYRIVIBRIO
  • RUMEN BACTERIUM
  • ORGANIZATION
  • REPLICATION
  • mobilizable cryptic plasmid
  • conjugation
  • open reading frames
  • plasmid mobilization
  • recombination
  • Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteroidaceae [06901] Ruminobacter amylophilus rumen bacterium, strain-NIAH-3
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Escherichia coli
  • Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000] bacteria
  • amino acids
  • DNA
  • proteins
  • small cryptic plasmid nucleotide sequence
  • 10052, Biochemistry methods - Nucleic acids, purines and pyrimidines
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10300, Replication, transcription, translation
  • 10506, Biophysics - Molecular properties and macromolecules
  • 13014, Metabolism - Nucleic acids, purines and pyrimidines
  • 14001, Digestive system - General and methods
  • 30500, Morphology and cytology of bacteria
  • 31000, Physiology and biochemistry of bacteria
  • 31500, Genetics of bacteria and viruses
  • 36002, Medical and clinical microbiology - Bacteriology
  • Biochemistry and Molecular Biophysics
  • AB004908 Genbank, EMBL, DDBJ 193234-13-8 nucleotide sequence
  • AB022866 Genbank, EMBL, DDBJ 225394-62-7 nucleotide sequence
  • Molecular Genetics

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