A small cryptic plasmid from Ruminobacter amylophilus NIAH-3 possesses functional mobilization properties

K. Ogata; T. Sekizaki; Rustam Aminov; K. Tajima; M. Nakamura; T. Nagamine; H. Matsui; Y. Benno

doi:10.1016/S0378-1097(99)00512-1

A small cryptic plasmid from Ruminobacter amylophilus NIAH-3 possesses functional mobilization properties

K. Ogata, T. Sekizaki, Rustam Aminov, K. Tajima, M. Nakamura, T. Nagamine, H. Matsui, Y. Benno

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Abstract

The complete nucleotide sequence of a small cryptic plasmid designated pRAO1, from the Gram-negative ruminal bacterium Ruminobacter amylophilus NIAH-3, was determined. The plasmid is a circular DNA molecule, 2140 bp in size, with a GC content of 40%. Computer-assisted analysis identified three open reading frames (ORFs), one of which, ORF3 (347 amino acids), displayed a high degree of amino acid identity with the Mob proteins involved in conjugative mobilization and interplasmid recombination of plasmids from Gram-positive bacteria. We proved the mobilization properties of pRAO1 in the Escherichia coli system using the coresident IncW broad-host-range conjugative plasmid R388. These data demonstrated, for the first time, the mobilization properties of small cryptic plasmids from Gram-negative inhabitants of the rumen. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Original language	English
Journal	FEMS Microbiology Letters
Volume	181
Issue number	1
Pages (from-to)	41-48
Number of pages	8
ISSN	0378-1097
DOIs	https://doi.org/10.1016/S0378-1097(99)00512-1
Publication status	Published - 1999
Externally published	Yes

Keywords

Amino Acid Sequence
Animals
Bacterial Proteins
Base Sequence
Conjugation, Genetic
DNA, Bacterial
DNA, Circular
DNA-Binding Proteins
Escherichia coli
Gammaproteobacteria
Gram-Negative Bacteria
Molecular Sequence Data
Phylogeny
Plasmids
Rumen
Sequence Analysis, DNA
Mob protein, Bacteria
article
DNA sequence
mobilization
nonhuman
nucleotide sequence
open reading frame
phylogeny
plasmid
priority journal
rumen microorganism
sequence analysis
Bacteria (microorganisms)
Negibacteria
Posibacteria
Ruminobacter amylophilus
Mobilizable cryptic plasmid
MICROBIOLOGY
COMPLETE NUCLEOTIDE-SEQUENCE
BACTEROIDES-AMYLOPHILUS
GENUS BUTYRIVIBRIO
RUMEN BACTERIUM
ORGANIZATION
REPLICATION
mobilizable cryptic plasmid
conjugation
open reading frames
plasmid mobilization
recombination
Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteroidaceae [06901] Ruminobacter amylophilus rumen bacterium, strain-NIAH-3
Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Escherichia coli
Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000] bacteria
amino acids
DNA
proteins
small cryptic plasmid nucleotide sequence
10052, Biochemistry methods - Nucleic acids, purines and pyrimidines
10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
10300, Replication, transcription, translation
10506, Biophysics - Molecular properties and macromolecules
13014, Metabolism - Nucleic acids, purines and pyrimidines
14001, Digestive system - General and methods
30500, Morphology and cytology of bacteria
31000, Physiology and biochemistry of bacteria
31500, Genetics of bacteria and viruses
36002, Medical and clinical microbiology - Bacteriology
Biochemistry and Molecular Biophysics
AB004908 Genbank, EMBL, DDBJ 193234-13-8 nucleotide sequence
AB022866 Genbank, EMBL, DDBJ 225394-62-7 nucleotide sequence
Molecular Genetics

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title = "A small cryptic plasmid from Ruminobacter amylophilus NIAH-3 possesses functional mobilization properties",

abstract = "The complete nucleotide sequence of a small cryptic plasmid designated pRAO1, from the Gram-negative ruminal bacterium Ruminobacter amylophilus NIAH-3, was determined. The plasmid is a circular DNA molecule, 2140 bp in size, with a GC content of 40%. Computer-assisted analysis identified three open reading frames (ORFs), one of which, ORF3 (347 amino acids), displayed a high degree of amino acid identity with the Mob proteins involved in conjugative mobilization and interplasmid recombination of plasmids from Gram-positive bacteria. We proved the mobilization properties of pRAO1 in the Escherichia coli system using the coresident IncW broad-host-range conjugative plasmid R388. These data demonstrated, for the first time, the mobilization properties of small cryptic plasmids from Gram-negative inhabitants of the rumen. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.",

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author = "K. Ogata and T. Sekizaki and Rustam Aminov and K. Tajima and M. Nakamura and T. Nagamine and H. Matsui and Y. Benno",

year = "1999",

doi = "10.1016/S0378-1097(99)00512-1",

language = "English",

volume = "181",

pages = "41--48",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

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TY - JOUR

T1 - A small cryptic plasmid from Ruminobacter amylophilus NIAH-3 possesses functional mobilization properties

AU - Ogata, K.

AU - Sekizaki, T.

AU - Aminov, Rustam

AU - Tajima, K.

AU - Nakamura, M.

AU - Nagamine, T.

AU - Matsui, H.

AU - Benno, Y.

PY - 1999

Y1 - 1999

N2 - The complete nucleotide sequence of a small cryptic plasmid designated pRAO1, from the Gram-negative ruminal bacterium Ruminobacter amylophilus NIAH-3, was determined. The plasmid is a circular DNA molecule, 2140 bp in size, with a GC content of 40%. Computer-assisted analysis identified three open reading frames (ORFs), one of which, ORF3 (347 amino acids), displayed a high degree of amino acid identity with the Mob proteins involved in conjugative mobilization and interplasmid recombination of plasmids from Gram-positive bacteria. We proved the mobilization properties of pRAO1 in the Escherichia coli system using the coresident IncW broad-host-range conjugative plasmid R388. These data demonstrated, for the first time, the mobilization properties of small cryptic plasmids from Gram-negative inhabitants of the rumen. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

AB - The complete nucleotide sequence of a small cryptic plasmid designated pRAO1, from the Gram-negative ruminal bacterium Ruminobacter amylophilus NIAH-3, was determined. The plasmid is a circular DNA molecule, 2140 bp in size, with a GC content of 40%. Computer-assisted analysis identified three open reading frames (ORFs), one of which, ORF3 (347 amino acids), displayed a high degree of amino acid identity with the Mob proteins involved in conjugative mobilization and interplasmid recombination of plasmids from Gram-positive bacteria. We proved the mobilization properties of pRAO1 in the Escherichia coli system using the coresident IncW broad-host-range conjugative plasmid R388. These data demonstrated, for the first time, the mobilization properties of small cryptic plasmids from Gram-negative inhabitants of the rumen. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

KW - Amino Acid Sequence

KW - Animals

KW - Bacterial Proteins

KW - Base Sequence

KW - Conjugation, Genetic

KW - DNA, Bacterial

KW - DNA, Circular

KW - DNA-Binding Proteins

KW - Escherichia coli

KW - Gammaproteobacteria

KW - Gram-Negative Bacteria

KW - Molecular Sequence Data

KW - Phylogeny

KW - Plasmids

KW - Rumen

KW - Sequence Analysis, DNA

KW - Mob protein, Bacteria

KW - article

KW - DNA sequence

KW - mobilization

KW - nonhuman

KW - nucleotide sequence

KW - open reading frame

KW - phylogeny

KW - plasmid

KW - priority journal

KW - rumen microorganism

KW - sequence analysis

KW - Bacteria (microorganisms)

KW - Negibacteria

KW - Posibacteria

KW - Ruminobacter amylophilus

KW - Mobilizable cryptic plasmid

KW - MICROBIOLOGY

KW - COMPLETE NUCLEOTIDE-SEQUENCE

KW - BACTEROIDES-AMYLOPHILUS

KW - GENUS BUTYRIVIBRIO

KW - RUMEN BACTERIUM

KW - ORGANIZATION

KW - REPLICATION

KW - mobilizable cryptic plasmid

KW - conjugation

KW - open reading frames

KW - plasmid mobilization

KW - recombination

KW - Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteroidaceae [06901] Ruminobacter amylophilus rumen bacterium, strain-NIAH-3

KW - Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Escherichia coli

KW - Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000] bacteria

KW - amino acids

KW - DNA

KW - proteins

KW - small cryptic plasmid nucleotide sequence

KW - 10052, Biochemistry methods - Nucleic acids, purines and pyrimidines

KW - 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines

KW - 10300, Replication, transcription, translation

KW - 10506, Biophysics - Molecular properties and macromolecules

KW - 13014, Metabolism - Nucleic acids, purines and pyrimidines

KW - 14001, Digestive system - General and methods

KW - 30500, Morphology and cytology of bacteria

KW - 31000, Physiology and biochemistry of bacteria

KW - 31500, Genetics of bacteria and viruses

KW - 36002, Medical and clinical microbiology - Bacteriology

KW - Biochemistry and Molecular Biophysics

KW - AB004908 Genbank, EMBL, DDBJ 193234-13-8 nucleotide sequence

KW - AB022866 Genbank, EMBL, DDBJ 225394-62-7 nucleotide sequence

KW - Molecular Genetics

U2 - 10.1016/S0378-1097(99)00512-1

DO - 10.1016/S0378-1097(99)00512-1

M3 - Journal article

SN - 0378-1097

VL - 181

SP - 41

EP - 48

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

A small cryptic plasmid from Ruminobacter amylophilus NIAH-3 possesses functional mobilization properties

Abstract

Keywords

Access to Document

OpenUrl availability

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Cite this