Lactone flavors with fruity, milky, coconut, and other aromas are widely used in the food and fragrance industries. Lactones are produced by chemical synthesis or by biotransformation of plant-sourced hydroxy fatty acids. We established a novel method to produce flavor lactones from abundant non-hydroxylated fatty acids using yeast cell factories. Oleaginous yeast Yarrowia lipolytica was engineered to perform hydroxylation of fatty acids and chain-shortening via β-oxidation to preferentially twelve or ten carbons. The strains could produce γ-dodecalactone from oleic acid and δ-decalactone from linoleic acid. Through metabolic engineering, the titer was improved 4-fold, and the final strain produced 282 mg/L γ-dodecalactone in a fed-batch bioreactor. The study paves the way for the production of lactones by fermentation of abundant fatty feedstocks.
- Flavor lactone
- Hydroxy fatty acids
- Yarrowia lipolytica
Marella, E. R., Dahlin, J., Dam, M. I., ter Horst, J., Christensen, H. B., Sudarsan, S., Wang, G., Holkenbrink, C., & Borodina, I. (Accepted/In press). A single-host fermentation process for the production of flavor lactones from non-hydroxylated fatty acids. Metabolic Engineering. https://doi.org/10.1016/j.ymben.2019.08.009