Abstract
For studies on iron absorption in man radioisotopes represent an easy and simple tool. However, measurement of the orbital electron emitting radioiron, Fe-55, in blood is difficult and insufficiently described in the literature. The present study describes a relatively simple method for simultaneous determination of Fe-55 and Fe-59 in blood, using a dry-ashing procedure and recrystallization of the remaining iron. The detection Limit of the method permits measurements of 0.1 Bq/ml blood thus allowing detection of Less than 1% absorption from a 40 kBq dose, which is ethically acceptable in humans. The overall recovery of radioiron from blood is more than 90%, and the coefficient of variation, as judged by the variation in the ratio Fe-55/Fe-59 is in the order of 4%. Combined with whole-body counting of 59Fe and direct gamma -counting of Fe-59 on blood samples, this method represents a sensitive method for studying the intestinal absorption of Fe-55 and Fe-59 in man and at the same time allows estimation of the amount of radioiron Located in the vascular compartment.
| Original language | English |
|---|---|
| Journal | Journal of Trace Elements in Medicine and Biology |
| Volume | 15 |
| Issue number | 1 |
| Pages (from-to) | 56-58 |
| ISSN | 0946-672X |
| DOIs | |
| Publication status | Published - 2001 |
Keywords
- protein
- lipd
- blood
- iron
- iron absorption
- non-heme-iron
- radioiron
- radioisotope