A simplified method for determination of radioactive iron in whole-blood samples

Klaus Bukhave, Anne Dorthe Sørensen, M. Hansen

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    For studies on iron absorption in man radioisotopes represent an easy and simple tool. However, measurement of the orbital electron emitting radioiron, Fe-55, in blood is difficult and insufficiently described in the literature. The present study describes a relatively simple method for simultaneous determination of Fe-55 and Fe-59 in blood, using a dry-ashing procedure and recrystallization of the remaining iron. The detection Limit of the method permits measurements of 0.1 Bq/ml blood thus allowing detection of Less than 1% absorption from a 40 kBq dose, which is ethically acceptable in humans. The overall recovery of radioiron from blood is more than 90%, and the coefficient of variation, as judged by the variation in the ratio Fe-55/Fe-59 is in the order of 4%. Combined with whole-body counting of 59Fe and direct gamma -counting of Fe-59 on blood samples, this method represents a sensitive method for studying the intestinal absorption of Fe-55 and Fe-59 in man and at the same time allows estimation of the amount of radioiron Located in the vascular compartment.
    Original languageEnglish
    JournalJournal of Trace Elements in Medicine and Biology
    Issue number1
    Pages (from-to)56-58
    Publication statusPublished - 2001


    • protein
    • lipd
    • blood
    • iron
    • iron absorption
    • non-heme-iron
    • radioiron
    • radioisotope

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