Abstract
A rapid and reliable method for purification of herpesvirus DNA from cell cultures is described. The method is based on the isolation of virus particles and/or nucleocapsids by differential centrifugation and exploits the solubilizing and denaturing capabilities of cesium trifluoroacetate during isopycnic centrifugation, so that phenol/chloroform extractions can be omitted. The method was used for the purification of DNA from several members of the Alfaherpesvirinae subfamily.
| Original language | English |
|---|---|
| Journal | Journal of Virological Methods |
| Volume | 37 |
| Issue number | 1 |
| Pages (from-to) | 99-102 |
| ISSN | 0166-0934 |
| DOIs | |
| Publication status | Published - 1992 |