A simple method for purification of herpesvirus DNA

Laurids Siig Christensen, Preben Normann

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A rapid and reliable method for purification of herpesvirus DNA from cell cultures is described. The method is based on the isolation of virus particles and/or nucleocapsids by differential centrifugation and exploits the solubilizing and denaturing capabilities of cesium trifluoroacetate during isopycnic centrifugation, so that phenol/chloroform extractions can be omitted. The method was used for the purification of DNA from several members of the Alfaherpesvirinae subfamily.
Original languageEnglish
JournalJournal of Virological Methods
Volume37
Issue number1
Pages (from-to)99-102
ISSN0166-0934
DOIs
Publication statusPublished - 1992

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