A Semi-Closed Device for Chromosome Spreading for Cytogenetic Analysis

Dorota Kwasny, Olga Mednova, Indumathi Vedarethinam, Maria Dimaki, Asli Silahtaroglu, Zeynep Tümer, Kristoffer Almdal, Winnie Edith Svendsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    308 Downloads (Pure)

    Abstract

    Metaphase chromosome spreading is the most crucial step required for successful karyotyping and FISH analysis. These two techniques are routinely used in cytogenetics to assess the chromosome abnormalities. The spreading process has been studied for years but it is still considered an art more than a science. The chromosome spreading greatly depends on the environmental conditions such as humidity and temperature, which govern the evaporation of fixative, in which the cells are suspended. The spreading is normally performed manually in ambient conditions on glass slides, which are hydrophilic, and thus allow for better quality spreads. Further cytogenetic analysis depends on the quality of the spreads, which is dependent on the skills of the personnel and is thus limited to laboratory settings. Here, we present a semi-closed microfluidic chip for preparation of the metaphase spreads on a glass and a Topasr substrate rendered more hydrophilic by oxygen plasma treatment coupled with photografting. The device consists of a microfluidic chamber with perfusion holes that facilitate the evaporation of fixative and reliable formation of the spreads. The usability of the chromosome spreads formed on the glass and the Topasr slide is tested by performing FISH analysis.
    Original languageEnglish
    JournalMicromachines
    Volume5
    Issue number2
    Pages (from-to)158-170
    ISSN2072-666X
    DOIs
    Publication statusPublished - 2014

    Bibliographical note

    Creative Commons Attribution Licence

    Cite this