Abstract
A sample holder for handling samples of protein for in-house X-ray powder diffraction (XRPD) analysis has been made and tested on lysozyme. The use of an integrated pinhole reduced the background, and good signal-to-noise ratios were obtained from only 7 l of sample, corresponding to approximately 2-3 mg of dry protein. The sample holder is further adaptable to X-ray absorption spectroscopy (XAS) measurements. Both XRPD and XAS at the Zn K-edge were tested with hexameric Zn insulin.
Original language | English |
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Journal | Journal of Applied Crystallography |
Volume | 44 |
Issue number | 6 |
Pages (from-to) | 1288-1290 |
ISSN | 0021-8898 |
DOIs | |
Publication status | Published - 2011 |
Bibliographical note
© 2011 International Union of CrystallographyKeywords
- Lysozyme
- In-house powder diffraction
- X-ray absorption spectroscopy
- Sample holders
- Insulin