A sample holder for in-house X-ray powder diffraction studies of protein powders

Christian Grundahl Frankær; Pernille Harris; Kenny Ståhl

doi:10.1107/S0021889811039057

A sample holder for in-house X-ray powder diffraction studies of protein powders

Christian Grundahl Frankær, Pernille Harris, Kenny Ståhl

Department of Chemistry

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Abstract

A sample holder for handling samples of protein for in-house X-ray powder diffraction (XRPD) analysis has been made and tested on lysozyme. The use of an integrated pinhole reduced the background, and good signal-to-noise ratios were obtained from only 7 l of sample, corresponding to approximately 2-3 mg of dry protein. The sample holder is further adaptable to X-ray absorption spectroscopy (XAS) measurements. Both XRPD and XAS at the Zn K-edge were tested with hexameric Zn insulin.

Original language	English
Journal	Journal of Applied Crystallography
Volume	44
Issue number	6
Pages (from-to)	1288-1290
ISSN	0021-8898
DOIs	https://doi.org/10.1107/S0021889811039057
Publication status	Published - 2011

Bibliographical note

Keywords

Lysozyme
In-house powder diffraction
X-ray absorption spectroscopy
Sample holders
Insulin

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title = "A sample holder for in-house X-ray powder diffraction studies of protein powders",

abstract = "A sample holder for handling samples of protein for in-house X-ray powder diffraction (XRPD) analysis has been made and tested on lysozyme. The use of an integrated pinhole reduced the background, and good signal-to-noise ratios were obtained from only 7 l of sample, corresponding to approximately 2-3 mg of dry protein. The sample holder is further adaptable to X-ray absorption spectroscopy (XAS) measurements. Both XRPD and XAS at the Zn K-edge were tested with hexameric Zn insulin.",

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AU - Harris, Pernille

AU - Ståhl, Kenny

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AB - A sample holder for handling samples of protein for in-house X-ray powder diffraction (XRPD) analysis has been made and tested on lysozyme. The use of an integrated pinhole reduced the background, and good signal-to-noise ratios were obtained from only 7 l of sample, corresponding to approximately 2-3 mg of dry protein. The sample holder is further adaptable to X-ray absorption spectroscopy (XAS) measurements. Both XRPD and XAS at the Zn K-edge were tested with hexameric Zn insulin.

KW - Lysozyme

KW - In-house powder diffraction

KW - X-ray absorption spectroscopy

KW - Sample holders

KW - Insulin

U2 - 10.1107/S0021889811039057

DO - 10.1107/S0021889811039057

M3 - Journal article

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JO - Journal of Applied Crystallography

JF - Journal of Applied Crystallography

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ER -

A sample holder for in-house X-ray powder diffraction studies of protein powders

Abstract

Bibliographical note

Keywords

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