A remote but significant sequence homology between glycoside hydrolase clan GH-H and glycoside hydrolase family GH 31

S. Janecek, Birte Svensson, E.A. MacGregor

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Although both the α-amylase super-family, i.e. the glycoside hydrolase (GH) clan GH-H (the GH families 13, 70 and 77), and family GH31 share some characteristics, their different catalytic machinery prevents classification of GH31 in clan GH-H. A significant but remote evolutionary relatedness is, however, proposed for clan GH-H with GH31. A sequence alignment, based on the idea that residues equivalent in the primordial catalytic GH-H/GH31 (β/α)8-barrel may not be found in the present-day GH-H and GH31 structures at strictly equivalent positions, shows remote sequence homologies covering β3, β4, β7 and β8 of the GH-H and GH31 (β/α)8-barrels. Structure comparison of GH13 α-amylase and GH31 α-xylosidase guided alignment of GH-H and GH31 members for construction of evolutionary trees. The closest sequence relationship displayed by GH31 is to GH77 of clan GH-H.
    Original languageEnglish
    JournalF E B S Letters
    Volume581
    Issue number7
    Pages (from-to)1261-1268
    ISSN0014-5793
    DOIs
    Publication statusPublished - 2007

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