A recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis

Louise von Gersdorff Jørgensen, Per Kania, Dagoberto Sepúlveda, Niels Lorenzen, Ansgar Stratmann, Kurt Buchmann

Research output: Contribution to conferencePosterResearchpeer-review

22 Downloads (Pure)

Abstract

New vaccine candidates were identified targeting the one celled parasite Ichthyophthirius multifiliis, which negatively affects aquaculture freshwater fish productions all over the world. In silico selection with the use of artificial intelligence identified several potential vaccine candidates and three of these were recombinantly expressed using E. coli and insect cells. Following a vaccine trial one protein (a so-called neurohypophysial n-terminal domain protein, #10) was found to induce moderate protection against I. multifiliis in rainbow trout (Oncorhynchus mykiss). To develop a highly protective heterologous vaccine we aim to combine #10 with a protective epitope from the already known homologous protective antigen Iag52b, which is a GPI-anchored cysteine rich surface protein. To be able to produce #10 at low costs, recombinant expression has been conducted in an eukaryotic host. Purified Iag52b does not induce immunity in fish without the use of adjuvants, thus the most potentially protective epitope of Iag52 was selected in silico and coupled to a virus-like particle. This coupling enables the epitope to be presented in a virus-like conformation, which theoretically should be immunogenic to the fish
Original languageEnglish
Publication date2019
Number of pages1
Publication statusPublished - 2019
EventThe 8th Congress of the Scandinavian-Baltic Society for Parasitology - Festauditoriet (the Main Lecture Hall, Frederiksberg, Denmark
Duration: 10 Oct 201911 Oct 2019
http://csbsp8evpc2019.eu

Conference

ConferenceThe 8th Congress of the Scandinavian-Baltic Society for Parasitology
LocationFestauditoriet (the Main Lecture Hall
CountryDenmark
CityFrederiksberg
Period10/10/201911/10/2019
Internet address

Cite this

Jørgensen, L. V. G., Kania, P., Sepúlveda, D., Lorenzen, N., Stratmann, A., & Buchmann, K. (2019). A recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis. Poster session presented at The 8th Congress of the Scandinavian-Baltic Society for Parasitology, Frederiksberg, Denmark.
Jørgensen, Louise von Gersdorff ; Kania, Per ; Sepúlveda, Dagoberto ; Lorenzen, Niels ; Stratmann, Ansgar ; Buchmann, Kurt. / A recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis. Poster session presented at The 8th Congress of the Scandinavian-Baltic Society for Parasitology, Frederiksberg, Denmark.1 p.
@conference{ce573de740e5422c95af1fffdb54a960,
title = "A recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis",
abstract = "New vaccine candidates were identified targeting the one celled parasite Ichthyophthirius multifiliis, which negatively affects aquaculture freshwater fish productions all over the world. In silico selection with the use of artificial intelligence identified several potential vaccine candidates and three of these were recombinantly expressed using E. coli and insect cells. Following a vaccine trial one protein (a so-called neurohypophysial n-terminal domain protein, #10) was found to induce moderate protection against I. multifiliis in rainbow trout (Oncorhynchus mykiss). To develop a highly protective heterologous vaccine we aim to combine #10 with a protective epitope from the already known homologous protective antigen Iag52b, which is a GPI-anchored cysteine rich surface protein. To be able to produce #10 at low costs, recombinant expression has been conducted in an eukaryotic host. Purified Iag52b does not induce immunity in fish without the use of adjuvants, thus the most potentially protective epitope of Iag52 was selected in silico and coupled to a virus-like particle. This coupling enables the epitope to be presented in a virus-like conformation, which theoretically should be immunogenic to the fish",
author = "J{\o}rgensen, {Louise von Gersdorff} and Per Kania and Dagoberto Sep{\'u}lveda and Niels Lorenzen and Ansgar Stratmann and Kurt Buchmann",
year = "2019",
language = "English",
note = "The 8th Congress of the Scandinavian-Baltic Society for Parasitology, SBSP ; Conference date: 10-10-2019 Through 11-10-2019",
url = "http://csbsp8evpc2019.eu",

}

Jørgensen, LVG, Kania, P, Sepúlveda, D, Lorenzen, N, Stratmann, A & Buchmann, K 2019, 'A recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis' The 8th Congress of the Scandinavian-Baltic Society for Parasitology, Frederiksberg, Denmark, 10/10/2019 - 11/10/2019, .

A recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis. / Jørgensen, Louise von Gersdorff; Kania, Per; Sepúlveda, Dagoberto; Lorenzen, Niels; Stratmann, Ansgar; Buchmann, Kurt.

2019. Poster session presented at The 8th Congress of the Scandinavian-Baltic Society for Parasitology, Frederiksberg, Denmark.

Research output: Contribution to conferencePosterResearchpeer-review

TY - CONF

T1 - A recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis

AU - Jørgensen, Louise von Gersdorff

AU - Kania, Per

AU - Sepúlveda, Dagoberto

AU - Lorenzen, Niels

AU - Stratmann, Ansgar

AU - Buchmann, Kurt

PY - 2019

Y1 - 2019

N2 - New vaccine candidates were identified targeting the one celled parasite Ichthyophthirius multifiliis, which negatively affects aquaculture freshwater fish productions all over the world. In silico selection with the use of artificial intelligence identified several potential vaccine candidates and three of these were recombinantly expressed using E. coli and insect cells. Following a vaccine trial one protein (a so-called neurohypophysial n-terminal domain protein, #10) was found to induce moderate protection against I. multifiliis in rainbow trout (Oncorhynchus mykiss). To develop a highly protective heterologous vaccine we aim to combine #10 with a protective epitope from the already known homologous protective antigen Iag52b, which is a GPI-anchored cysteine rich surface protein. To be able to produce #10 at low costs, recombinant expression has been conducted in an eukaryotic host. Purified Iag52b does not induce immunity in fish without the use of adjuvants, thus the most potentially protective epitope of Iag52 was selected in silico and coupled to a virus-like particle. This coupling enables the epitope to be presented in a virus-like conformation, which theoretically should be immunogenic to the fish

AB - New vaccine candidates were identified targeting the one celled parasite Ichthyophthirius multifiliis, which negatively affects aquaculture freshwater fish productions all over the world. In silico selection with the use of artificial intelligence identified several potential vaccine candidates and three of these were recombinantly expressed using E. coli and insect cells. Following a vaccine trial one protein (a so-called neurohypophysial n-terminal domain protein, #10) was found to induce moderate protection against I. multifiliis in rainbow trout (Oncorhynchus mykiss). To develop a highly protective heterologous vaccine we aim to combine #10 with a protective epitope from the already known homologous protective antigen Iag52b, which is a GPI-anchored cysteine rich surface protein. To be able to produce #10 at low costs, recombinant expression has been conducted in an eukaryotic host. Purified Iag52b does not induce immunity in fish without the use of adjuvants, thus the most potentially protective epitope of Iag52 was selected in silico and coupled to a virus-like particle. This coupling enables the epitope to be presented in a virus-like conformation, which theoretically should be immunogenic to the fish

M3 - Poster

ER -

Jørgensen LVG, Kania P, Sepúlveda D, Lorenzen N, Stratmann A, Buchmann K. A recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis. 2019. Poster session presented at The 8th Congress of the Scandinavian-Baltic Society for Parasitology, Frederiksberg, Denmark.