Abstract
| Original language | English |
|---|---|
| Publication date | 2019 |
| Number of pages | 1 |
| Publication status | Published - 2019 |
| Event | The 8th Congress of the Scandinavian-Baltic Society for Parasitology - Festauditoriet (the Main Lecture Hall, Frederiksberg, Denmark Duration: 10 Oct 2019 → 11 Oct 2019 http://csbsp8evpc2019.eu |
Conference
| Conference | The 8th Congress of the Scandinavian-Baltic Society for Parasitology |
|---|---|
| Location | Festauditoriet (the Main Lecture Hall |
| Country | Denmark |
| City | Frederiksberg |
| Period | 10/10/2019 → 11/10/2019 |
| Internet address |
Cite this
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A recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis. / Jørgensen, Louise von Gersdorff; Kania, Per; Sepúlveda, Dagoberto; Lorenzen, Niels; Stratmann, Ansgar; Buchmann, Kurt.
2019. Poster session presented at The 8th Congress of the Scandinavian-Baltic Society for Parasitology, Frederiksberg, Denmark.Research output: Contribution to conference › Poster › Research › peer-review
TY - CONF
T1 - A recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis
AU - Jørgensen, Louise von Gersdorff
AU - Kania, Per
AU - Sepúlveda, Dagoberto
AU - Lorenzen, Niels
AU - Stratmann, Ansgar
AU - Buchmann, Kurt
PY - 2019
Y1 - 2019
N2 - New vaccine candidates were identified targeting the one celled parasite Ichthyophthirius multifiliis, which negatively affects aquaculture freshwater fish productions all over the world. In silico selection with the use of artificial intelligence identified several potential vaccine candidates and three of these were recombinantly expressed using E. coli and insect cells. Following a vaccine trial one protein (a so-called neurohypophysial n-terminal domain protein, #10) was found to induce moderate protection against I. multifiliis in rainbow trout (Oncorhynchus mykiss). To develop a highly protective heterologous vaccine we aim to combine #10 with a protective epitope from the already known homologous protective antigen Iag52b, which is a GPI-anchored cysteine rich surface protein. To be able to produce #10 at low costs, recombinant expression has been conducted in an eukaryotic host. Purified Iag52b does not induce immunity in fish without the use of adjuvants, thus the most potentially protective epitope of Iag52 was selected in silico and coupled to a virus-like particle. This coupling enables the epitope to be presented in a virus-like conformation, which theoretically should be immunogenic to the fish
AB - New vaccine candidates were identified targeting the one celled parasite Ichthyophthirius multifiliis, which negatively affects aquaculture freshwater fish productions all over the world. In silico selection with the use of artificial intelligence identified several potential vaccine candidates and three of these were recombinantly expressed using E. coli and insect cells. Following a vaccine trial one protein (a so-called neurohypophysial n-terminal domain protein, #10) was found to induce moderate protection against I. multifiliis in rainbow trout (Oncorhynchus mykiss). To develop a highly protective heterologous vaccine we aim to combine #10 with a protective epitope from the already known homologous protective antigen Iag52b, which is a GPI-anchored cysteine rich surface protein. To be able to produce #10 at low costs, recombinant expression has been conducted in an eukaryotic host. Purified Iag52b does not induce immunity in fish without the use of adjuvants, thus the most potentially protective epitope of Iag52 was selected in silico and coupled to a virus-like particle. This coupling enables the epitope to be presented in a virus-like conformation, which theoretically should be immunogenic to the fish
M3 - Poster
ER -