A reappraisal of Aspergillus section Nidulantes with descriptions of two new sterigmatocystin-producing species

Vit Hubka, Alena Nováková, Stephen W. Peterson, Jens Christian Frisvad, František Sklenář, Tetsuhiro Matsuzawa, Alena Kubátová, Miroslav Kolařík

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Aspergillus section Nidulantes is a speciose group of microscopic fungi whose species are important in indoor air quality, food spoilage, mycotoxin production and human pathogenicity. We assembled as many species from the section as possible with either type specimens or protologues for analysis. DNA sequences were obtained from up to four loci to conduct phylogenetic analysis of sect. Nidulantes and other species in subgenus Nidulantes. Comparisons of phenetic characters were made. Our goal was to recognize monophyletic taxa for section Nidulantes as well as the other taxa in the subgenus. Phylogenetic analysis indicated that sections Versicolores and Aenei formed subclades in the larger clade containing the type species of section Nidulantes. In order to maintain monophyly in section Nidulantes, we synonymized sects. Versicolores and Aenei with sect. Nidulantes. In place of sections, we have identified six subclades in sect. Nidulantes that also have shared phenetic character sets that allow for easier morphological identification of species. Earlier reports showed that standard β-tubulin primers can occasionally amplify the tubC gene rather than the benA gene they were designed for. We show that codon usage analysis can easily distinguish between these paralogues, and we provide primer pair options to allow benA amplifications. Two new sect. Nidulantes species are described from European caves, A. croceus (Spain) and A. askiburgiensis (Czech Republic). The analysis of exometabolites showed that both species are able to produce the mycotoxin sterigmatocystin and some additional compounds.
    Original languageEnglish
    JournalPlant Systematics and Evolution
    Volume302
    Issue number9
    Pages (from-to)1267-1299
    Number of pages33
    ISSN0378-2697
    DOIs
    Publication statusPublished - 2016

    Keywords

    • Aspergillus nidulans
    • Codon usage
    • Emericella
    • Mycotoxins
    • Paralogous genes
    • Subgeneric classification of Aspergillus

    Cite this

    Hubka, Vit ; Nováková, Alena ; Peterson, Stephen W. ; Frisvad, Jens Christian ; Sklenář, František ; Matsuzawa, Tetsuhiro ; Kubátová, Alena ; Kolařík, Miroslav. / A reappraisal of Aspergillus section Nidulantes with descriptions of two new sterigmatocystin-producing species. In: Plant Systematics and Evolution. 2016 ; Vol. 302, No. 9. pp. 1267-1299.
    @article{0f185ebebe874525a42ad2b192710dae,
    title = "A reappraisal of Aspergillus section Nidulantes with descriptions of two new sterigmatocystin-producing species",
    abstract = "Aspergillus section Nidulantes is a speciose group of microscopic fungi whose species are important in indoor air quality, food spoilage, mycotoxin production and human pathogenicity. We assembled as many species from the section as possible with either type specimens or protologues for analysis. DNA sequences were obtained from up to four loci to conduct phylogenetic analysis of sect. Nidulantes and other species in subgenus Nidulantes. Comparisons of phenetic characters were made. Our goal was to recognize monophyletic taxa for section Nidulantes as well as the other taxa in the subgenus. Phylogenetic analysis indicated that sections Versicolores and Aenei formed subclades in the larger clade containing the type species of section Nidulantes. In order to maintain monophyly in section Nidulantes, we synonymized sects. Versicolores and Aenei with sect. Nidulantes. In place of sections, we have identified six subclades in sect. Nidulantes that also have shared phenetic character sets that allow for easier morphological identification of species. Earlier reports showed that standard β-tubulin primers can occasionally amplify the tubC gene rather than the benA gene they were designed for. We show that codon usage analysis can easily distinguish between these paralogues, and we provide primer pair options to allow benA amplifications. Two new sect. Nidulantes species are described from European caves, A. croceus (Spain) and A. askiburgiensis (Czech Republic). The analysis of exometabolites showed that both species are able to produce the mycotoxin sterigmatocystin and some additional compounds.",
    keywords = "Aspergillus nidulans, Codon usage, Emericella, Mycotoxins, Paralogous genes, Subgeneric classification of Aspergillus",
    author = "Vit Hubka and Alena Nov{\'a}kov{\'a} and Peterson, {Stephen W.} and Frisvad, {Jens Christian} and František Sklen{\'a}ř and Tetsuhiro Matsuzawa and Alena Kub{\'a}tov{\'a} and Miroslav Kolař{\'i}k",
    year = "2016",
    doi = "10.1007/s00606-016-1331-5",
    language = "English",
    volume = "302",
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    Hubka, V, Nováková, A, Peterson, SW, Frisvad, JC, Sklenář, F, Matsuzawa, T, Kubátová, A & Kolařík, M 2016, 'A reappraisal of Aspergillus section Nidulantes with descriptions of two new sterigmatocystin-producing species', Plant Systematics and Evolution, vol. 302, no. 9, pp. 1267-1299. https://doi.org/10.1007/s00606-016-1331-5

    A reappraisal of Aspergillus section Nidulantes with descriptions of two new sterigmatocystin-producing species. / Hubka, Vit; Nováková, Alena; Peterson, Stephen W.; Frisvad, Jens Christian; Sklenář, František; Matsuzawa, Tetsuhiro; Kubátová, Alena; Kolařík, Miroslav.

    In: Plant Systematics and Evolution, Vol. 302, No. 9, 2016, p. 1267-1299.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - A reappraisal of Aspergillus section Nidulantes with descriptions of two new sterigmatocystin-producing species

    AU - Hubka, Vit

    AU - Nováková, Alena

    AU - Peterson, Stephen W.

    AU - Frisvad, Jens Christian

    AU - Sklenář, František

    AU - Matsuzawa, Tetsuhiro

    AU - Kubátová, Alena

    AU - Kolařík, Miroslav

    PY - 2016

    Y1 - 2016

    N2 - Aspergillus section Nidulantes is a speciose group of microscopic fungi whose species are important in indoor air quality, food spoilage, mycotoxin production and human pathogenicity. We assembled as many species from the section as possible with either type specimens or protologues for analysis. DNA sequences were obtained from up to four loci to conduct phylogenetic analysis of sect. Nidulantes and other species in subgenus Nidulantes. Comparisons of phenetic characters were made. Our goal was to recognize monophyletic taxa for section Nidulantes as well as the other taxa in the subgenus. Phylogenetic analysis indicated that sections Versicolores and Aenei formed subclades in the larger clade containing the type species of section Nidulantes. In order to maintain monophyly in section Nidulantes, we synonymized sects. Versicolores and Aenei with sect. Nidulantes. In place of sections, we have identified six subclades in sect. Nidulantes that also have shared phenetic character sets that allow for easier morphological identification of species. Earlier reports showed that standard β-tubulin primers can occasionally amplify the tubC gene rather than the benA gene they were designed for. We show that codon usage analysis can easily distinguish between these paralogues, and we provide primer pair options to allow benA amplifications. Two new sect. Nidulantes species are described from European caves, A. croceus (Spain) and A. askiburgiensis (Czech Republic). The analysis of exometabolites showed that both species are able to produce the mycotoxin sterigmatocystin and some additional compounds.

    AB - Aspergillus section Nidulantes is a speciose group of microscopic fungi whose species are important in indoor air quality, food spoilage, mycotoxin production and human pathogenicity. We assembled as many species from the section as possible with either type specimens or protologues for analysis. DNA sequences were obtained from up to four loci to conduct phylogenetic analysis of sect. Nidulantes and other species in subgenus Nidulantes. Comparisons of phenetic characters were made. Our goal was to recognize monophyletic taxa for section Nidulantes as well as the other taxa in the subgenus. Phylogenetic analysis indicated that sections Versicolores and Aenei formed subclades in the larger clade containing the type species of section Nidulantes. In order to maintain monophyly in section Nidulantes, we synonymized sects. Versicolores and Aenei with sect. Nidulantes. In place of sections, we have identified six subclades in sect. Nidulantes that also have shared phenetic character sets that allow for easier morphological identification of species. Earlier reports showed that standard β-tubulin primers can occasionally amplify the tubC gene rather than the benA gene they were designed for. We show that codon usage analysis can easily distinguish between these paralogues, and we provide primer pair options to allow benA amplifications. Two new sect. Nidulantes species are described from European caves, A. croceus (Spain) and A. askiburgiensis (Czech Republic). The analysis of exometabolites showed that both species are able to produce the mycotoxin sterigmatocystin and some additional compounds.

    KW - Aspergillus nidulans

    KW - Codon usage

    KW - Emericella

    KW - Mycotoxins

    KW - Paralogous genes

    KW - Subgeneric classification of Aspergillus

    U2 - 10.1007/s00606-016-1331-5

    DO - 10.1007/s00606-016-1331-5

    M3 - Journal article

    VL - 302

    SP - 1267

    EP - 1299

    JO - Plant Systematics and Evolution

    JF - Plant Systematics and Evolution

    SN - 0378-2697

    IS - 9

    ER -