Abstract
A real-time PCR assay was developed based on a 18 1 -bp fragment of the recently cloned per gene, including an internal amplification control (124 bp), for the detection of Yersinia enterocolitica 0:9 (Ye 0:9). The validation included 48 Ye 0:9, 33 Y enterocolitica non-0:9 and 35 other closely-related bacterial strains, containing per gene homologies. The assay was specific for the Ye 0:9 tested, the detection limit was 1-10 genome copies of purified DNA and amplification efficiency was between 90.5-103%, indicating a linear regression throughout the detection window.
| Original language | English |
|---|---|
| Journal | Journal of Microbiological Methods |
| Volume | 63 |
| Issue number | 2 |
| Pages (from-to) | 151-156 |
| ISSN | 0167-7012 |
| Publication status | Published - 2005 |
Keywords
- enterocolitica
- O : 9
- real-time
- Yersinia
- PCR