A practical approach to producing the single-arm linear dextrin, a chimeric glucosaccharide containing an (α-1 → 4) linked portion at the nonreducing end of an (α-1 → 6) glucochain

Yazhen Wu, Xiaoxiao Li, Zhengyu Jin, Birte Svensson, Yuxiang Bai*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

How to improve the solubility of linear dextrins (LD) and retain their characteristic helix amphiphilic cavities with flexible embedding capability, is a question worth exploring without adding new chemical groups. The strategy presented in this study is to attach a highly flexible (α-1 → 6) glucochain at the reducing end of LD by preparing a new type of dextrin, referred to as single-arm linear dextrin (SLD). In the actual synthesis, an (α-1 → 6) linked oligosaccharide of DP 10.7 (PDI = 1.28) was formed by extension of glucose units onto sucrose (2 M) by using L940W mutant of the glucansucrase GTF180-ΔN firstly. Next using γ-CD as glucosylation donor γ-CGTase extended this (α-1 → 6) glucochain with (α-1 → 4) bonds. SLD is a chimeric glucosaccharide comprising an (α-1 → 4) linked part (DP 10.5) attached to the nonreducing end of an (α-1 → 6) glucochain as verified by enzyme fingerprinting and 1H NMR. Furthermore, SLD was validated to show greatly improved solubility and dispersibility of resveratrol in water, as indicated by a 3.12-fold enhancement over the solubility in the presence of 0.014 M SLD. This study provided a new strategy for solving the solubility problem of LD and opens possibilities for new design of the fine structure of starch-like materials.
Original languageEnglish
Article number120520
JournalCarbohydrate Polymers
Volume305
Number of pages11
ISSN0144-8617
DOIs
Publication statusPublished - 2023

Keywords

  • Single-arm linear dextrin
  • Isomalto-ogliosaccharides
  • γ-CD
  • γ-CGTase
  • Chimeric glucosaccharide
  • Solubility

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