In the Middle Danube Basin, Quaternary deposits are widely distributed in the Vojvodina region where they cover about 95% of the area. Major research during the last two decades has been focused on loess deposits in the Vojvodina region. During this period, loess in the Vojvodina region has become one of the most important Pleistocene European continental climatic and environmental records. Here we present the dating results of 15 samples taken from the Nosak loess-palaeosol sequence in northeastern Serbia in order to establish a chronology over the last three glacial–interglacial cycles. We use the pIRIR290 signal of the 4–11 μm polymineral grains. The calculated ages are within the error limits partially consistent with the proposed multi-millennial chronostratigraphy for Serbian loess. The average mass accumulation rate for the last three glacial–interglacial cycles is 265 g m−2 a−1, which is in agreement with the values of most sites in the Carpathian Basin. Our results indicate a highly variable deposition rate of loess, especially during the MIS 3 and MIS 6 stages, which is contrary to most studies conducted in Serbia where linear sedimentation rates were assumed.