Abstract
Comprehensive knowledge of intracellular
biochemistry is needed to accurately understand, model,
and manipulate metabolism for industrial and therapeutic
applications. Quantitative metabolomics has been driven
by advances in analytical instrumentation and can add
valuable knowledge to the understanding of intracellular
metabolism. Liquid chromatography coupled to mass
spectrometry (LC–MS and LC–MS/MS) has become a
reliable means with which to quantify a multitude of intracellular
metabolites in parallel with great specificity and
accuracy. This work details a method that builds and extends
upon existing reverse phase ion-paring liquid chromatography
methods for separation and detection of polar
and anionic compounds that comprise key nodes of intracellular
metabolism by optimizing pH and solvent composition.
In addition, the presented method utilizes multiple
scan types provided by hybrid instrumentation to improve
confidence in compound identification. The developed
method was validated for a broad coverage of polar and
anionic metabolites of intracellular metabolism
Original language | English |
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Journal | Metabolomics |
Volume | 11 |
Issue number | 5 |
Pages (from-to) | 1338-1350 |
Number of pages | 13 |
ISSN | 1573-3882 |
DOIs | |
Publication status | Published - 2015 |
Keywords
- Reverse-phase ion-pairing liquid chromatography mass spectrometry
- Intracellular metabolism
- Absolute quantification
- Metabolomics