A pentavalent vaccine for rainbow trout in Danish aquaculture

Moonika H Marana*, Dagoberto Andres Sepulveda Araneda, Defang Chen, Azmi Al-Jubury, Rzgar M Jaafar, Per W Kania, Niels Henrik Henriksen, Bjørn Krossøy, Inger Dalsgaard, Niels Lorenzen, Kurt Buchmann

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Mariculture in Denmark is based on production of rainbow trout grown two years in fresh water followed by one growth season in sea cages. Although the majority of rainbow trout are vaccinated against the most serious bacterial pathogens - Aeromonas salmonicida subsp. salmonicida, Vibrio anguillarum and Yersinia ruckeri, by the use of commercially available vaccines, disease outbreaks requiring treatment with antibiotics still occur. The present study tested the potential of a new experimental multicomponent vaccine that is based on local bacterial strains, isolated from rainbow trout in Danish waters, and thus custom-designed for Danish rainbow trout mariculture. The vaccination with the multicomponent vaccine resulted in protection against three relevant bacterial diseases (yersiniosis, furunculosis, vibriosis) under experimental conditions. We showed that i.p. injection of the vaccine induced specific antibody responses in trout against the different bacterial antigens and regulated expression of genes encoding SAA, C3, IL-1β, IL-6, IL-8, IgD and MHCII.
Original languageEnglish
JournalFish and Shellfish Immunology
Volume88
Pages (from-to)344-351
ISSN1050-4648
DOIs
Publication statusPublished - 2019

Keywords

  • Aeromonas salmonicida subsp. salmonicida
  • Aquaculture
  • Rainbow trout
  • Vaccination
  • Vibrio anguillarum
  • Yersinia ruckeri

Cite this

Marana, M. H., Sepulveda Araneda, D. A., Chen, D., Al-Jubury, A., Jaafar, R. M., Kania, P. W., ... Buchmann, K. (2019). A pentavalent vaccine for rainbow trout in Danish aquaculture. Fish and Shellfish Immunology, 88, 344-351. https://doi.org/10.1016/j.fsi.2019.03.001
Marana, Moonika H ; Sepulveda Araneda, Dagoberto Andres ; Chen, Defang ; Al-Jubury, Azmi ; Jaafar, Rzgar M ; Kania, Per W ; Henriksen, Niels Henrik ; Krossøy, Bjørn ; Dalsgaard, Inger ; Lorenzen, Niels ; Buchmann, Kurt. / A pentavalent vaccine for rainbow trout in Danish aquaculture. In: Fish and Shellfish Immunology. 2019 ; Vol. 88. pp. 344-351.
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title = "A pentavalent vaccine for rainbow trout in Danish aquaculture",
abstract = "Mariculture in Denmark is based on production of rainbow trout grown two years in fresh water followed by one growth season in sea cages. Although the majority of rainbow trout are vaccinated against the most serious bacterial pathogens - Aeromonas salmonicida subsp. salmonicida, Vibrio anguillarum and Yersinia ruckeri, by the use of commercially available vaccines, disease outbreaks requiring treatment with antibiotics still occur. The present study tested the potential of a new experimental multicomponent vaccine that is based on local bacterial strains, isolated from rainbow trout in Danish waters, and thus custom-designed for Danish rainbow trout mariculture. The vaccination with the multicomponent vaccine resulted in protection against three relevant bacterial diseases (yersiniosis, furunculosis, vibriosis) under experimental conditions. We showed that i.p. injection of the vaccine induced specific antibody responses in trout against the different bacterial antigens and regulated expression of genes encoding SAA, C3, IL-1β, IL-6, IL-8, IgD and MHCII.",
keywords = "Aeromonas salmonicida subsp. salmonicida, Aquaculture, Rainbow trout, Vaccination, Vibrio anguillarum, Yersinia ruckeri",
author = "Marana, {Moonika H} and {Sepulveda Araneda}, {Dagoberto Andres} and Defang Chen and Azmi Al-Jubury and Jaafar, {Rzgar M} and Kania, {Per W} and Henriksen, {Niels Henrik} and Bj{\o}rn Kross{\o}y and Inger Dalsgaard and Niels Lorenzen and Kurt Buchmann",
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Marana, MH, Sepulveda Araneda, DA, Chen, D, Al-Jubury, A, Jaafar, RM, Kania, PW, Henriksen, NH, Krossøy, B, Dalsgaard, I, Lorenzen, N & Buchmann, K 2019, 'A pentavalent vaccine for rainbow trout in Danish aquaculture', Fish and Shellfish Immunology, vol. 88, pp. 344-351. https://doi.org/10.1016/j.fsi.2019.03.001

A pentavalent vaccine for rainbow trout in Danish aquaculture. / Marana, Moonika H; Sepulveda Araneda, Dagoberto Andres; Chen, Defang; Al-Jubury, Azmi; Jaafar, Rzgar M; Kania, Per W; Henriksen, Niels Henrik; Krossøy, Bjørn; Dalsgaard, Inger; Lorenzen, Niels; Buchmann, Kurt.

In: Fish and Shellfish Immunology, Vol. 88, 2019, p. 344-351.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - A pentavalent vaccine for rainbow trout in Danish aquaculture

AU - Marana, Moonika H

AU - Sepulveda Araneda, Dagoberto Andres

AU - Chen, Defang

AU - Al-Jubury, Azmi

AU - Jaafar, Rzgar M

AU - Kania, Per W

AU - Henriksen, Niels Henrik

AU - Krossøy, Bjørn

AU - Dalsgaard, Inger

AU - Lorenzen, Niels

AU - Buchmann, Kurt

PY - 2019

Y1 - 2019

N2 - Mariculture in Denmark is based on production of rainbow trout grown two years in fresh water followed by one growth season in sea cages. Although the majority of rainbow trout are vaccinated against the most serious bacterial pathogens - Aeromonas salmonicida subsp. salmonicida, Vibrio anguillarum and Yersinia ruckeri, by the use of commercially available vaccines, disease outbreaks requiring treatment with antibiotics still occur. The present study tested the potential of a new experimental multicomponent vaccine that is based on local bacterial strains, isolated from rainbow trout in Danish waters, and thus custom-designed for Danish rainbow trout mariculture. The vaccination with the multicomponent vaccine resulted in protection against three relevant bacterial diseases (yersiniosis, furunculosis, vibriosis) under experimental conditions. We showed that i.p. injection of the vaccine induced specific antibody responses in trout against the different bacterial antigens and regulated expression of genes encoding SAA, C3, IL-1β, IL-6, IL-8, IgD and MHCII.

AB - Mariculture in Denmark is based on production of rainbow trout grown two years in fresh water followed by one growth season in sea cages. Although the majority of rainbow trout are vaccinated against the most serious bacterial pathogens - Aeromonas salmonicida subsp. salmonicida, Vibrio anguillarum and Yersinia ruckeri, by the use of commercially available vaccines, disease outbreaks requiring treatment with antibiotics still occur. The present study tested the potential of a new experimental multicomponent vaccine that is based on local bacterial strains, isolated from rainbow trout in Danish waters, and thus custom-designed for Danish rainbow trout mariculture. The vaccination with the multicomponent vaccine resulted in protection against three relevant bacterial diseases (yersiniosis, furunculosis, vibriosis) under experimental conditions. We showed that i.p. injection of the vaccine induced specific antibody responses in trout against the different bacterial antigens and regulated expression of genes encoding SAA, C3, IL-1β, IL-6, IL-8, IgD and MHCII.

KW - Aeromonas salmonicida subsp. salmonicida

KW - Aquaculture

KW - Rainbow trout

KW - Vaccination

KW - Vibrio anguillarum

KW - Yersinia ruckeri

U2 - 10.1016/j.fsi.2019.03.001

DO - 10.1016/j.fsi.2019.03.001

M3 - Journal article

VL - 88

SP - 344

EP - 351

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

SN - 1050-4648

ER -