A Passive Microfluidic Device for Chemotaxis Studies

Research output: Contribution to journalJournal article – Annual report year: 2019Researchpeer-review

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  • Author: Coluccio, Maria Laura

    Magna Græcia University, Italy

  • Author: D'Attimo, Maria Antonia

    Magna Græcia University, Italy

  • Author: Cristiani, Costanza Maria

    Magna Græcia University, Italy

  • Author: Candeloro, Patrizio

    Magna Græcia University, Italy

  • Author: Parrotta, Elvira

    Magna Græcia University, Italy

  • Author: Dattola, Elisabetta

    Magna Græcia University, Italy

  • Author: Guzzi, Francesco

    Magna Græcia University, Italy

  • Author: Cuda, Giovanni

    Magna Græcia University, Italy

  • Author: Lamanna, Ernesto

    Magna Græcia University, Italy

  • Author: Carbone, Ennio

    Magna Græcia University, Italy

  • Author: Krühne, Ulrich

    PROSYS - Process and Systems Engineering Centre, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Søltofts Plads, 2800, Kgs. Lyngby, Denmark

  • Author: Di Fabrizio, Enzo

    Magna Græcia University, Italy

  • Author: Perozziello, Gerardo

    Magna Græcia University, Italy

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This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO2. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µm/min, comparable with the rates present in scientific literature.
Original languageEnglish
Article number551
JournalMicromachines
Volume10
Issue number8
Number of pages11
ISSN2072-666X
DOIs
Publication statusPublished - 2019

Bibliographical note

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • Chemotaxis, Diffusion, Mini incubator, Passive microfluidic device

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