A Passive Microfluidic Device for Chemotaxis Studies

Maria Laura Coluccio; Maria Antonia D'Attimo; Costanza Maria Cristiani; Patrizio Candeloro; Elvira Parrotta; Elisabetta Dattola; Francesco Guzzi; Giovanni Cuda; Ernesto Lamanna; Ennio Carbone; Ulrich Krühne; Enzo Di Fabrizio; Gerardo Perozziello

doi:10.3390/mi10080551

A Passive Microfluidic Device for Chemotaxis Studies

Maria Laura Coluccio, Maria Antonia D'Attimo, Costanza Maria Cristiani, Patrizio Candeloro, Elvira Parrotta, Elisabetta Dattola, Francesco Guzzi, Giovanni Cuda, Ernesto Lamanna, Ennio Carbone, Ulrich Krühne, Enzo Di Fabrizio, Gerardo Perozziello^*

^*Corresponding author for this work

Magna Græcia University

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO₂. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µm/min, comparable with the rates present in scientific literature.

Original language	English
Article number	551
Journal	Micromachines
Volume	10
Issue number	8
Number of pages	11
ISSN	2072-666X
DOIs	https://doi.org/10.3390/mi10080551
Publication status	Published - 2019

Bibliographical note

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Keywords

Chemotaxis
Diffusion
Mini incubator
Passive microfluidic device

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Micromachines 10 00551Final published version, 2.02 MB

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abstract = "This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO2. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µm/min, comparable with the rates present in scientific literature.",

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AU - Coluccio, Maria Laura

AU - D'Attimo, Maria Antonia

AU - Cristiani, Costanza Maria

AU - Candeloro, Patrizio

AU - Parrotta, Elvira

AU - Dattola, Elisabetta

AU - Guzzi, Francesco

AU - Cuda, Giovanni

AU - Lamanna, Ernesto

AU - Carbone, Ennio

AU - Krühne, Ulrich

AU - Di Fabrizio, Enzo

AU - Perozziello, Gerardo

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N2 - This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO2. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µm/min, comparable with the rates present in scientific literature.

AB - This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO2. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µm/min, comparable with the rates present in scientific literature.

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KW - Passive microfluidic device

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A Passive Microfluidic Device for Chemotaxis Studies

Abstract

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