A Passive Microfluidic Device for Chemotaxis Studies

Maria Laura Coluccio, Maria Antonia D'Attimo, Costanza Maria Cristiani, Patrizio Candeloro, Elvira Parrotta, Elisabetta Dattola, Francesco Guzzi, Giovanni Cuda, Ernesto Lamanna, Ennio Carbone, Ulrich Krühne, Enzo Di Fabrizio, Gerardo Perozziello*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO2. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µm/min, comparable with the rates present in scientific literature.
Original languageEnglish
Article number551
JournalMicromachines
Volume10
Issue number8
Number of pages11
ISSN2072-666X
DOIs
Publication statusPublished - 2019

Bibliographical note

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Keywords

  • Chemotaxis
  • Diffusion
  • Mini incubator
  • Passive microfluidic device

Cite this

Coluccio, M. L., D'Attimo, M. A., Cristiani, C. M., Candeloro, P., Parrotta, E., Dattola, E., ... Perozziello, G. (2019). A Passive Microfluidic Device for Chemotaxis Studies. Micromachines, 10(8), [551]. https://doi.org/10.3390/mi10080551
Coluccio, Maria Laura ; D'Attimo, Maria Antonia ; Cristiani, Costanza Maria ; Candeloro, Patrizio ; Parrotta, Elvira ; Dattola, Elisabetta ; Guzzi, Francesco ; Cuda, Giovanni ; Lamanna, Ernesto ; Carbone, Ennio ; Krühne, Ulrich ; Di Fabrizio, Enzo ; Perozziello, Gerardo. / A Passive Microfluidic Device for Chemotaxis Studies. In: Micromachines. 2019 ; Vol. 10, No. 8.
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abstract = "This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5{\%} in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5{\%} of CO2. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µm/min, comparable with the rates present in scientific literature.",
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author = "Coluccio, {Maria Laura} and D'Attimo, {Maria Antonia} and Cristiani, {Costanza Maria} and Patrizio Candeloro and Elvira Parrotta and Elisabetta Dattola and Francesco Guzzi and Giovanni Cuda and Ernesto Lamanna and Ennio Carbone and Ulrich Kr{\"u}hne and {Di Fabrizio}, Enzo and Gerardo Perozziello",
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Coluccio, ML, D'Attimo, MA, Cristiani, CM, Candeloro, P, Parrotta, E, Dattola, E, Guzzi, F, Cuda, G, Lamanna, E, Carbone, E, Krühne, U, Di Fabrizio, E & Perozziello, G 2019, 'A Passive Microfluidic Device for Chemotaxis Studies', Micromachines, vol. 10, no. 8, 551. https://doi.org/10.3390/mi10080551

A Passive Microfluidic Device for Chemotaxis Studies. / Coluccio, Maria Laura; D'Attimo, Maria Antonia; Cristiani, Costanza Maria; Candeloro, Patrizio; Parrotta, Elvira; Dattola, Elisabetta; Guzzi, Francesco; Cuda, Giovanni; Lamanna, Ernesto; Carbone, Ennio; Krühne, Ulrich; Di Fabrizio, Enzo; Perozziello, Gerardo.

In: Micromachines, Vol. 10, No. 8, 551, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - A Passive Microfluidic Device for Chemotaxis Studies

AU - Coluccio, Maria Laura

AU - D'Attimo, Maria Antonia

AU - Cristiani, Costanza Maria

AU - Candeloro, Patrizio

AU - Parrotta, Elvira

AU - Dattola, Elisabetta

AU - Guzzi, Francesco

AU - Cuda, Giovanni

AU - Lamanna, Ernesto

AU - Carbone, Ennio

AU - Krühne, Ulrich

AU - Di Fabrizio, Enzo

AU - Perozziello, Gerardo

N1 - This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PY - 2019

Y1 - 2019

N2 - This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO2. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µm/min, comparable with the rates present in scientific literature.

AB - This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO2. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µm/min, comparable with the rates present in scientific literature.

KW - Chemotaxis

KW - Diffusion

KW - Mini incubator

KW - Passive microfluidic device

U2 - 10.3390/mi10080551

DO - 10.3390/mi10080551

M3 - Journal article

VL - 10

JO - Micromachines

JF - Micromachines

SN - 2072-666X

IS - 8

M1 - 551

ER -

Coluccio ML, D'Attimo MA, Cristiani CM, Candeloro P, Parrotta E, Dattola E et al. A Passive Microfluidic Device for Chemotaxis Studies. Micromachines. 2019;10(8). 551. https://doi.org/10.3390/mi10080551