A novel temperature control method for shortening thermal cycling time to achieve rapid polymerase chain reaction (PCR) in a disposable polymer microfluidic device.

Minqiang Bu, Ivan R. Perch-Nielsen, Karen Skotte Sørensen, Julia Skov, Sun Yi, Dang Duong Bang, Michael E. Pedersen, Mikkel Fougt Hansen, Anders Wolff

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Abstract

We present a new temperature control method capable of effectively shortening the thermal cycling time of polymerase chain reaction (PCR) in a disposable polymer microfluidic device with external heater and temperature sensor. The method employs optimized temperature overshooting and undershooting steps to achieve a rapid ramping between the temperature steps for DNA denaturation, annealing and extension. The temperature dynamics within the microfluidic PCR chamber was characterized and the overshooting and undershooting parameters were optimized using the temperature dependent fluorescence signal from Rhodamine B. The method was validated with PCR amplification of mecA gene (162 bp) from Methicillin-resistant Staphylococcus aureus bacterium (MRSA), where the time for 30 cycles was reduced from 50 min (without over- and undershooting) to 20 min.
Original languageEnglish
Publication date2012
Number of pages4
Publication statusPublished - 2012
Event23rd Micromechanics And Microsystems Europe Workshop - Ilmenau, Germany
Duration: 9 Sep 201212 Dec 2012
Conference number: 23

Conference

Conference23rd Micromechanics And Microsystems Europe Workshop
Number23
CountryGermany
CityIlmenau
Period09/09/201212/12/2012

Keywords

  • Temperature control method
  • Polymerase chain reaction (PCR)
  • Polymer microfluidic device

Cite this

Bu, M., R. Perch-Nielsen, I., Sørensen, K. S., Skov, J., Yi, S., Bang, D. D., ... Wolff, A. (2012). A novel temperature control method for shortening thermal cycling time to achieve rapid polymerase chain reaction (PCR) in a disposable polymer microfluidic device.. Paper presented at 23rd Micromechanics And Microsystems Europe Workshop, Ilmenau, Germany.