Abstract
Despite recent and substantial advances in biosensing, information and communication, and Lab-on-Chip (LoC) technologies, the success of Point-of-Care (PoC) diagnostics and monitoring systems is still challenged by stringent requirements for robustness, cost-effectiveness, and system integration.
The pitfalls of PoC system adoption can be addressed early in the system design phase. They require a multidisciplinary design approach supported by systems engineering tools and methods. Considering this, we here present both a model and an implementation of a simple and rapid calibration scheme for instrument-based PoC blood biomarker analysis systems. Motivated by the complexity of associating high-accuracy biosensing using silicon nanowire field effect transistors with ease of use for the PoC system user, we propose a novel one-step, multipoint calibration method for LoC-based systems. Our approach specifically addresses the important interfaces between a novel microfluidic unit to integrate the sensor array and a mobile-device hardware accessory. A multi-point calibration curve is obtained by generating a defined set of reference concentrations from a single input. By consecutively splitting the flow perpendicular to the diffusion interface only one mixing step is required for each of the generated calibration solutions. This results in a compact design with a very small footprint of the microfluidic layout.
The pitfalls of PoC system adoption can be addressed early in the system design phase. They require a multidisciplinary design approach supported by systems engineering tools and methods. Considering this, we here present both a model and an implementation of a simple and rapid calibration scheme for instrument-based PoC blood biomarker analysis systems. Motivated by the complexity of associating high-accuracy biosensing using silicon nanowire field effect transistors with ease of use for the PoC system user, we propose a novel one-step, multipoint calibration method for LoC-based systems. Our approach specifically addresses the important interfaces between a novel microfluidic unit to integrate the sensor array and a mobile-device hardware accessory. A multi-point calibration curve is obtained by generating a defined set of reference concentrations from a single input. By consecutively splitting the flow perpendicular to the diffusion interface only one mixing step is required for each of the generated calibration solutions. This results in a compact design with a very small footprint of the microfluidic layout.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of Lab-on-a-Chip European Congress 2014 |
| Publication date | 2014 |
| Publication status | Published - 2014 |
| Event | Lab-on-a-Chip European Congress 2014 - Berlin, Germany Duration: 10 Mar 2014 → 11 Mar 2014 Conference number: 6 http://selectbiosciences.com/conferences/index.aspx?conf=LOACEC2014 |
Conference
| Conference | Lab-on-a-Chip European Congress 2014 |
|---|---|
| Number | 6 |
| Country/Territory | Germany |
| City | Berlin |
| Period | 10/03/2014 → 11/03/2014 |
| Internet address |