A novel single-step, multipoint calibration method for instrumented Lab-on-Chip systems

Andrea Pfreundt, François Patou, Azeem Zulfiqar, Maria Dimaki, Jan Madsen, Winnie Edith Svendsen

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review


Despite recent and substantial advances in biosensing, information and communication, and Lab-on-Chip (LoC) technologies, the success of Point-of-Care (PoC) diagnostics and monitoring systems is still challenged by stringent requirements for robustness, cost-effectiveness, and system integration.
The pitfalls of PoC system adoption can be addressed early in the system design phase. They require a multidisciplinary design approach supported by systems engineering tools and methods. Considering this, we here present both a model and an implementation of a simple and rapid calibration scheme for instrument-based PoC blood biomarker analysis systems. Motivated by the complexity of associating high-accuracy biosensing using silicon nanowire field effect transistors with ease of use for the PoC system user, we propose a novel one-step, multipoint calibration method for LoC-based systems. Our approach specifically addresses the important interfaces between a novel microfluidic unit to integrate the sensor array and a mobile-device hardware accessory. A multi-point calibration curve is obtained by generating a defined set of reference concentrations from a single input. By consecutively splitting the flow perpendicular to the diffusion interface only one mixing step is required for each of the generated calibration solutions. This results in a compact design with a very small footprint of the microfluidic layout.
Original languageEnglish
Title of host publicationProceedings of Lab-on-a-Chip European Congress 2014
Publication date2014
Publication statusPublished - 2014
EventLab-on-a-Chip European Congress 2014 - Berlin, Germany
Duration: 10 Mar 201411 Mar 2014
Conference number: 6


ConferenceLab-on-a-Chip European Congress 2014
Internet address


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