TY - JOUR
T1 - A novel PEG-mediated approach to entrap hemoglobin (Hb) within ZIF-8 nanoparticles
T2 - Balancing crystalline structure, Hb content and functionality
AU - Coll-Satue, Clara
AU - Rubio-Huertas, Marta
AU - Ducrot, Aurelie
AU - Norkute, Evita
AU - Liu, Xiaoli
AU - Ebrahim, Fatmah Mish
AU - Smit, Berend
AU - Thulstrup, Peter Waaben
AU - Hosta-Rigau, Leticia
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024
Y1 - 2024
N2 - Hemoglobin (Hb)-based oxygen carriers are investigated as a potential alternative or supplement to regular blood transfusions, particularly in critical and life-threatening scenarios. These include situations like severe trauma in remote areas, battlefield conditions, instances where blood transfusion is not feasible due to compatibility concerns, or when patients decline transfusions based on religious beliefs. This study introduces a novel method utilizing poly(ethylene glycol) (PEG) to entrap Hb within ZIF-8 nanoparticles (i.e., Hb@ZIF-8 NPs). Through meticulous screening, we achieved Hb@ZIF-8 NPs with a record-high Hb concentration of 34 mg mL−1. These NPs, sized at 168 nm, displayed exceptional properties: a remarkable 95 % oxyhemoglobin content, excellent encapsulation efficiency of 85 %, and resistance to Hb oxidation into methemoglobin (metHb). The addition of PEG emerged as a crucial factor amplifying Hb entrapment within ZIF-8, especially at higher Hb concentrations, reaching an unprecedented 34 mg mL−1. Importantly, PEG exhibited a protective effect, preventing metHb conversion in Hb@ZIF-8 NPs at elevated Hb concentrations.
AB - Hemoglobin (Hb)-based oxygen carriers are investigated as a potential alternative or supplement to regular blood transfusions, particularly in critical and life-threatening scenarios. These include situations like severe trauma in remote areas, battlefield conditions, instances where blood transfusion is not feasible due to compatibility concerns, or when patients decline transfusions based on religious beliefs. This study introduces a novel method utilizing poly(ethylene glycol) (PEG) to entrap Hb within ZIF-8 nanoparticles (i.e., Hb@ZIF-8 NPs). Through meticulous screening, we achieved Hb@ZIF-8 NPs with a record-high Hb concentration of 34 mg mL−1. These NPs, sized at 168 nm, displayed exceptional properties: a remarkable 95 % oxyhemoglobin content, excellent encapsulation efficiency of 85 %, and resistance to Hb oxidation into methemoglobin (metHb). The addition of PEG emerged as a crucial factor amplifying Hb entrapment within ZIF-8, especially at higher Hb concentrations, reaching an unprecedented 34 mg mL−1. Importantly, PEG exhibited a protective effect, preventing metHb conversion in Hb@ZIF-8 NPs at elevated Hb concentrations.
KW - Blood substitutes
KW - Hemoglobin
KW - Hemoglobin-based oxygen carriers
KW - Metal-organic frameworks
KW - ZIF-8
U2 - 10.1016/j.bioadv.2024.213953
DO - 10.1016/j.bioadv.2024.213953
M3 - Journal article
C2 - 39029206
AN - SCOPUS:85198755082
SN - 2772-9508
VL - 163
JO - Biomaterials Advances
JF - Biomaterials Advances
M1 - 213953
ER -