A novel passive microfluidic device for preprocessing whole blood for point of care diagnostics

Pranjul Jaykumar Shah; Maria Dimaki; Winnie Edith Svendsen

doi:10.1109/SENSOR.2009.5285475

A novel passive microfluidic device for preprocessing whole blood for point of care diagnostics

Pranjul Jaykumar Shah
, Maria Dimaki
, Winnie Edith Svendsen

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

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Abstract

A novel strategy to sort the cells of interest (White Blood Cells (leukocytes)) by selectively lysing the Red Blood Cells (erythrocytes) in a miniaturized microfluidic device is presented. Various methods to lyse cells on a chip exist i.e. electrical, mechanical, chemical and thermal but they need integration of electrodes, traps, reservoirs, heaters, etc which is often difficult at microscale [1 – 4]. On the other hand, FACSlyse protocol uses only osmotic pressure to lyse erythrocytes allowing further isolation of leukocytes. This motivated us to develop a novel herringbone based lyser which works on the principle of mixing whole blood with pure water in time controlled manner to lyse erythrocytes osmotically on a chip.

Original language	English
Title of host publication	International Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009.
Publisher	IEEE
Publication date	2009
ISBN (Electronic)	978-1-4244-4193-8
DOIs	https://doi.org/10.1109/SENSOR.2009.5285475
Publication status	Published - 2009
Event	15th International Solid-State Sensors, Actuators and Microsystems Conference (Transducers 2009) - Denver,CO, United States Duration: 21 Jun 2009 → 25 Jun 2009 Conference number: 15

Conference

Conference	15th International Solid-State Sensors, Actuators and Microsystems Conference (Transducers 2009)
Number	15
Country/Territory	United States
City	Denver,CO
Period	21/06/2009 → 25/06/2009

Bibliographical note

Copyright: 2009 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE

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@inproceedings{afb7bbcc72254b988f90159a9d2a2fac,

title = "A novel passive microfluidic device for preprocessing whole blood for point of care diagnostics",

abstract = "A novel strategy to sort the cells of interest (White Blood Cells (leukocytes)) by selectively lysing the Red Blood Cells (erythrocytes) in a miniaturized microfluidic device is presented. Various methods to lyse cells on a chip exist i.e. electrical, mechanical, chemical and thermal but they need integration of electrodes, traps, reservoirs, heaters, etc which is often difficult at microscale [1 – 4]. On the other hand, FACSlyse protocol uses only osmotic pressure to lyse erythrocytes allowing further isolation of leukocytes. This motivated us to develop a novel herringbone based lyser which works on the principle of mixing whole blood with pure water in time controlled manner to lyse erythrocytes osmotically on a chip.",

author = "Shah, \{Pranjul Jaykumar\} and Maria Dimaki and Svendsen, \{Winnie Edith\}",

note = "Copyright: 2009 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE; 15th International Solid-State Sensors, Actuators and Microsystems Conference (Transducers 2009), Transducers 2009 ; Conference date: 21-06-2009 Through 25-06-2009",

year = "2009",

doi = "10.1109/SENSOR.2009.5285475",

language = "English",

booktitle = "International Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009.",

publisher = "IEEE",

address = "United States",

}

Shah, PJ, Dimaki, M & Svendsen, WE 2009, A novel passive microfluidic device for preprocessing whole blood for point of care diagnostics. in International Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009.. IEEE, 15th International Solid-State Sensors, Actuators and Microsystems Conference (Transducers 2009), Denver,CO, United States, 21/06/2009. https://doi.org/10.1109/SENSOR.2009.5285475

A novel passive microfluidic device for preprocessing whole blood for point of care diagnostics. / Shah, Pranjul Jaykumar; Dimaki, Maria ; Svendsen, Winnie Edith.
International Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009.. IEEE, 2009.

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

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A novel passive microfluidic device for preprocessing whole blood for point of care diagnostics

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