A novel passive microfluidic device for preprocessing whole blood for point of care diagnostics

Pranjul Jaykumar Shah, Maria Dimaki, Winnie Edith Svendsen

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    Abstract

    A novel strategy to sort the cells of interest (White Blood Cells (leukocytes)) by selectively lysing the Red Blood Cells (erythrocytes) in a miniaturized microfluidic device is presented. Various methods to lyse cells on a chip exist i.e. electrical, mechanical, chemical and thermal but they need integration of electrodes, traps, reservoirs, heaters, etc which is often difficult at microscale [1 – 4]. On the other hand, FACSlyse protocol uses only osmotic pressure to lyse erythrocytes allowing further isolation of leukocytes. This motivated us to develop a novel herringbone based lyser which works on the principle of mixing whole blood with pure water in time controlled manner to lyse erythrocytes osmotically on a chip.
    Original languageEnglish
    Title of host publicationInternational Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009.
    PublisherIEEE
    Publication date2009
    ISBN (Electronic)978-1-4244-4193-8
    DOIs
    Publication statusPublished - 2009
    Event15th International Solid-State Sensors, Actuators and Microsystems Conference (Transducers 2009) - Denver,CO, United States
    Duration: 21 Jun 200925 Jun 2009
    Conference number: 15

    Conference

    Conference15th International Solid-State Sensors, Actuators and Microsystems Conference (Transducers 2009)
    Number15
    Country/TerritoryUnited States
    CityDenver,CO
    Period21/06/200925/06/2009

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