A Novel in Vivo Angiogenesis Mouse Model

Thomas Wittenborn, Jens V. Nygaard, Michael R. Horsman, Thomas Vorup-Jensen, Jorgen Kjems, Troels Thim, Thomas Nielsen, Esben Kjær Unmack Larsen, Erling Falk

Research output: Contribution to journalConference articleResearchpeer-review

Abstract

Purpose: To establish a reproducable, functional and fully vascularized angiogenesis model in mice for subsequent evaluation of leaky vessels associated with neovascularization. Materials and Methods: A biocompatible Poly-Capro-Lactone (PCL) scaffold was implanted subcutaneously in mice, and the development and progression of angiogenesis was evaluated up to 6 weeks after implantation. Mice were euthanized, sponges immersion fixed and paraffinembedded, and HE stained sections prepared for microscopic evaluation. Figure: Representative slides showing how new microvessels (angiogenesis) develop and progressively penetrate through the scaffold. The skin of the mouse is included for orientation. Results: Very little angiogenesis was seen within the scaffold after 1 week. During the subsequent weeks, the scaffold became progressively occupied by microvessel-containing new tissue. No or very limited extravasation of erythrocytes was seen. Within 5–6 weeks the implant was fully vascularized. Conclusion perspectives: We have developed a functional angiogenesis model capable of fully vascularizing the implant within 6 weeks. Histologic examination reveals few if any intra-implant hemorrhages, but Dynamic Contrast Enhanced (DCE)-MRI will elucidate if there are leaky vessels present in the scaffold. High resolution MRI and vessel density will also be assessed to fully characterize this model.
Original languageEnglish
JournalARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
Volume29
Issue number7
Pages (from-to)E95-E95
ISSN1079-5642
Publication statusPublished - 2009
Externally publishedYes
Event10th Annual Conference on Arteriosclerosis, Thrombosis and Vascular Biology - Omni Shoreham Hotel, Washington, United States
Duration: 29 Apr 20091 May 2009
Conference number: 10

Conference

Conference10th Annual Conference on Arteriosclerosis, Thrombosis and Vascular Biology
Number10
LocationOmni Shoreham Hotel
CountryUnited States
CityWashington
Period29/04/200901/05/2009

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