A novel fluorescent in situ hybridization technique for detection of Rickettsia spp. in archival samples

Claus Bo Svendsen; Mette Boye; Carsten Struve; Karen A. Krogfelt

doi:10.1016/j.mimet.2008.10.012

A novel fluorescent in situ hybridization technique for detection of Rickettsia spp. in archival samples

Claus Bo Svendsen, Mette Boye, Carsten Struve, Karen A. Krogfelt

Statens Serum Institut

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Ricekttsia was found without problems of cross-reactions with bacterial species shown to cross-react serologically.

Original language	English
Journal	Journal of Microbiological Methods
Volume	76
Issue number	3
Pages (from-to)	301-304
ISSN	0167-7012
DOIs	https://doi.org/10.1016/j.mimet.2008.10.012
Publication status	Published - 2009

Keywords

Fluorescence
In situ hybridization
Archival samples
Rickettsia

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10.1016/j.mimet.2008.10.012

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title = "A novel fluorescent in situ hybridization technique for detection of Rickettsia spp. in archival samples",

abstract = "A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Ricekttsia was found without problems of cross-reactions with bacterial species shown to cross-react serologically.",

keywords = "Fluorescence, In situ hybridization, Archival samples, Rickettsia",

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T1 - A novel fluorescent in situ hybridization technique for detection of Rickettsia spp. in archival samples

AU - Svendsen, Claus Bo

AU - Boye, Mette

AU - Struve, Carsten

AU - Krogfelt, Karen A.

PY - 2009

Y1 - 2009

N2 - A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Ricekttsia was found without problems of cross-reactions with bacterial species shown to cross-react serologically.

AB - A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Ricekttsia was found without problems of cross-reactions with bacterial species shown to cross-react serologically.

KW - Fluorescence

KW - In situ hybridization

KW - Archival samples

KW - Rickettsia

U2 - 10.1016/j.mimet.2008.10.012

DO - 10.1016/j.mimet.2008.10.012

M3 - Journal article

C2 - 19007824

SN - 0167-7012

VL - 76

SP - 301

EP - 304

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 3

ER -

A novel fluorescent in situ hybridization technique for detection of Rickettsia spp. in archival samples

Abstract

Keywords

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