We developed a high-sensitive platform to monior multiple hybridization events in real time. By creating a microoptical array in a polymeric chip, the system combine the excellent discriminative power of supercritical angle fluorescence (SAF) microscopy with high-throughput capabilities of microarrays. The micro-optical array is easy to fabricate, and exhibits significantly improved analytical performance. It has a potential to become a basic tool for applications such as gene expression or single nucleotide polymorphism (SNP) detection.
|Number of pages||3|
|Publication status||Published - 2012|
|Event||16th International Conference of Miniaturized Systems for Chemistry and Life Sciences - Okinawa Convention Center, Okinawa, Japan|
Duration: 28 Oct 2012 → 1 Nov 2012
Conference number: 16
|Conference||16th International Conference of Miniaturized Systems for Chemistry and Life Sciences|
|Location||Okinawa Convention Center|
|Period||28/10/2012 → 01/11/2012|
- Supercritical angle fluorescence
- DNA microarray
- Real-time monitoring
Yi, S., Perch-Nielsen, I. R., Wang, Z., Bang, D. D., & Wolff, A. (2012). A novel detection platform for parallel monitoring of DNA hybridization with high sensitivity and specificity. Paper presented at 16th International Conference of Miniaturized Systems for Chemistry and Life Sciences, Okinawa, Japan.