A novel detection platform for parallel monitoring of DNA hybridization with high sensitivity and specificity

Sun Yi, Ivan R. Perch-Nielsen, Zhenyu Wang, Dang Duong Bang, Anders Wolff

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Abstract

We developed a high-sensitive platform to monior multiple hybridization events in real time. By creating a microoptical array in a polymeric chip, the system combine the excellent discriminative power of supercritical angle fluorescence (SAF) microscopy with high-throughput capabilities of microarrays. The micro-optical array is easy to fabricate, and exhibits significantly improved analytical performance. It has a potential to become a basic tool for applications such as gene expression or single nucleotide polymorphism (SNP) detection.
Original languageEnglish
Publication date2012
Number of pages3
Publication statusPublished - 2012
Event16th International Conference of Miniaturized Systems for Chemistry and Life Sciences - Okinawa Convention Center, Okinawa, Japan
Duration: 28 Oct 20121 Nov 2012
Conference number: 16
http://www.microtas12.org/

Conference

Conference16th International Conference of Miniaturized Systems for Chemistry and Life Sciences
Number16
LocationOkinawa Convention Center
CountryJapan
CityOkinawa
Period28/10/201201/11/2012
Internet address

Keywords

  • Supercritical angle fluorescence
  • Micro-optics
  • DNA microarray
  • Real-time monitoring

Cite this

Yi, S., Perch-Nielsen, I. R., Wang, Z., Bang, D. D., & Wolff, A. (2012). A novel detection platform for parallel monitoring of DNA hybridization with high sensitivity and specificity. Paper presented at 16th International Conference of Miniaturized Systems for Chemistry and Life Sciences, Okinawa, Japan.