A novel closed system bubble column photobioreactor for detailed characterisation of micro- and macroalgal growth

Susan Løvstad Holdt, L. Christensen, J. J. L. Iversen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Growth of the marine microalga Tetraselmis striata Butcher and the macroalga Chondrus crispus Stackhouse was investigated in batch cultures in a closed system bubble column photobioreactor. A laboratory cultivation system was constructed that allowed online monitoring of pH and dissolved oxygen tension and was used for characterization of photoautotrophic growth. Carbon dioxide addition regulated pH and was used to optimise irradiance. Oxygen was removed from the system by addition of hydrogen over a palladium catalyst to quantify oxygen production. In addition, the bubble column photobioreactor was suited for cultivation of algae due to fast gas-to-liquid mass transfer (kLa) and fast mixing provided by split and dual sparging. Specific growth rates (SGRs) were measured using both offline and online measurements. The latter was possible, because rectilinear correlation was observed between carbon dioxide addition and optical density, which shows that carbon dioxide addition may be used as an indirect measurement of microalgal biomass (x). The slope of the rectilinear fit of ln (dx/dt) as a function of the time (t) then revealed the SGR. These determinations revealed detailed information about changes in growth with up to three different SGRs in the different batch cultures of both micro- and macroalgae. The maximum SGRs found by online determination were 0.13 h-1 for T. striata and 0.12 day-1 for C. crispus. We have developed and described a system and presented some data handling tools that provide new information about growth kinetics of algae. © 2013 Springer Science+Business Media Dordrecht.
Original languageEnglish
JournalJournal of Applied Phycology
Volume26
Issue number2
Pages (from-to)825-835
Number of pages11
ISSN0921-8971
DOIs
Publication statusPublished - 2014

Keywords

  • Characteristic mixing time
  • Chondrus crispus
  • kLa
  • Photosynthetic quotient
  • Specific growth rate
  • Tetraselmis striata

Cite this

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title = "A novel closed system bubble column photobioreactor for detailed characterisation of micro- and macroalgal growth",
abstract = "Growth of the marine microalga Tetraselmis striata Butcher and the macroalga Chondrus crispus Stackhouse was investigated in batch cultures in a closed system bubble column photobioreactor. A laboratory cultivation system was constructed that allowed online monitoring of pH and dissolved oxygen tension and was used for characterization of photoautotrophic growth. Carbon dioxide addition regulated pH and was used to optimise irradiance. Oxygen was removed from the system by addition of hydrogen over a palladium catalyst to quantify oxygen production. In addition, the bubble column photobioreactor was suited for cultivation of algae due to fast gas-to-liquid mass transfer (kLa) and fast mixing provided by split and dual sparging. Specific growth rates (SGRs) were measured using both offline and online measurements. The latter was possible, because rectilinear correlation was observed between carbon dioxide addition and optical density, which shows that carbon dioxide addition may be used as an indirect measurement of microalgal biomass (x). The slope of the rectilinear fit of ln (dx/dt) as a function of the time (t) then revealed the SGR. These determinations revealed detailed information about changes in growth with up to three different SGRs in the different batch cultures of both micro- and macroalgae. The maximum SGRs found by online determination were 0.13 h-1 for T. striata and 0.12 day-1 for C. crispus. We have developed and described a system and presented some data handling tools that provide new information about growth kinetics of algae. {\circledC} 2013 Springer Science+Business Media Dordrecht.",
keywords = "Characteristic mixing time, Chondrus crispus, kLa, Photosynthetic quotient, Specific growth rate, Tetraselmis striata",
author = "Holdt, {Susan L{\o}vstad} and L. Christensen and Iversen, {J. J. L.}",
year = "2014",
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A novel closed system bubble column photobioreactor for detailed characterisation of micro- and macroalgal growth. / Holdt, Susan Løvstad; Christensen, L.; Iversen, J. J. L.

In: Journal of Applied Phycology, Vol. 26, No. 2, 2014, p. 825-835.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - A novel closed system bubble column photobioreactor for detailed characterisation of micro- and macroalgal growth

AU - Holdt, Susan Løvstad

AU - Christensen, L.

AU - Iversen, J. J. L.

PY - 2014

Y1 - 2014

N2 - Growth of the marine microalga Tetraselmis striata Butcher and the macroalga Chondrus crispus Stackhouse was investigated in batch cultures in a closed system bubble column photobioreactor. A laboratory cultivation system was constructed that allowed online monitoring of pH and dissolved oxygen tension and was used for characterization of photoautotrophic growth. Carbon dioxide addition regulated pH and was used to optimise irradiance. Oxygen was removed from the system by addition of hydrogen over a palladium catalyst to quantify oxygen production. In addition, the bubble column photobioreactor was suited for cultivation of algae due to fast gas-to-liquid mass transfer (kLa) and fast mixing provided by split and dual sparging. Specific growth rates (SGRs) were measured using both offline and online measurements. The latter was possible, because rectilinear correlation was observed between carbon dioxide addition and optical density, which shows that carbon dioxide addition may be used as an indirect measurement of microalgal biomass (x). The slope of the rectilinear fit of ln (dx/dt) as a function of the time (t) then revealed the SGR. These determinations revealed detailed information about changes in growth with up to three different SGRs in the different batch cultures of both micro- and macroalgae. The maximum SGRs found by online determination were 0.13 h-1 for T. striata and 0.12 day-1 for C. crispus. We have developed and described a system and presented some data handling tools that provide new information about growth kinetics of algae. © 2013 Springer Science+Business Media Dordrecht.

AB - Growth of the marine microalga Tetraselmis striata Butcher and the macroalga Chondrus crispus Stackhouse was investigated in batch cultures in a closed system bubble column photobioreactor. A laboratory cultivation system was constructed that allowed online monitoring of pH and dissolved oxygen tension and was used for characterization of photoautotrophic growth. Carbon dioxide addition regulated pH and was used to optimise irradiance. Oxygen was removed from the system by addition of hydrogen over a palladium catalyst to quantify oxygen production. In addition, the bubble column photobioreactor was suited for cultivation of algae due to fast gas-to-liquid mass transfer (kLa) and fast mixing provided by split and dual sparging. Specific growth rates (SGRs) were measured using both offline and online measurements. The latter was possible, because rectilinear correlation was observed between carbon dioxide addition and optical density, which shows that carbon dioxide addition may be used as an indirect measurement of microalgal biomass (x). The slope of the rectilinear fit of ln (dx/dt) as a function of the time (t) then revealed the SGR. These determinations revealed detailed information about changes in growth with up to three different SGRs in the different batch cultures of both micro- and macroalgae. The maximum SGRs found by online determination were 0.13 h-1 for T. striata and 0.12 day-1 for C. crispus. We have developed and described a system and presented some data handling tools that provide new information about growth kinetics of algae. © 2013 Springer Science+Business Media Dordrecht.

KW - Characteristic mixing time

KW - Chondrus crispus

KW - kLa

KW - Photosynthetic quotient

KW - Specific growth rate

KW - Tetraselmis striata

U2 - 10.1007/s10811-013-0190-5

DO - 10.1007/s10811-013-0190-5

M3 - Journal article

VL - 26

SP - 825

EP - 835

JO - Journal of Applied Phycology

JF - Journal of Applied Phycology

SN - 0921-8971

IS - 2

ER -