Growth of the marine microalgae Tetraselmis striata Butcher and macroalgae Chondrus crispus Stackhouse was investigated in batch cultures in a closed system bubble column photobioreactor. A laboratory cultivation system was constructed that allowed on-line monitoring of temperature, pH and dissolved oxygen tension and was intended for characterization of photoautotrophic growth. Regulation of temperature was done by cooling and carbon dioxide additions regulated pH. The carbon dioxide addition rate was used to optimize irradiance and thereby optimizing growth conditions. Photosynthetically produced oxygen was catalytically removed from the closed system by addition of hydrogen over a palladium catalyst to avoid photorespiration and to quantify oxygen production. In addition, the bubble column photobioreactor was well suited for cultivation of algae due to fast gas to liquid mass transfer (kLa) and fast mixing provided by split and dual sparging. Specific growth rates (SGRs) were measured using both off-line and on-line measurements. The latter was possible, because linear correlation was observed between carbon dioxide addition and optical density, which proves that carbon dioxide addition may be used as an indirect measurement of biomass (x). The slope of the rectilinear fit of ln (dx/dt) as function of the time (t) then revealed the SGR. This allowed on-line determination of the SGR, and these determinations revealed detailed information about changes in growth with up to three different SGRs in the different batch cultures of both micro and macroalgae. The algal SGR is normally considered to be constant. The maximum SGRs found by on-line determination were 0.13 hr-1 for T. striata and 0.12 d-1 for C. crispus. During batch cultivation growth stoichiometry was determined and photosynthetic quotients and carbon mass balances were used to validate the system. We have developed a system and presented some data handling tools that provides new information about the growth kinetic of algae.
|Number of pages||1|
|Publication status||Published - 2013|
|Event||21st International Seaweed Symposium - Bali, Indonesia|
Duration: 21 Apr 2013 → 26 Apr 2013
Conference number: 21
|Conference||21st International Seaweed Symposium|
|Period||21/04/2013 → 26/04/2013|