A novel class of CoA-transferase involved in short-chain fatty acid metabolism in butyrate-producing human colonic bacteria

Cedric Charrier, Gary J. Duncan, Martin D. Reid, Garry J. Rucklidge, Donna Henderson, Pauline Young, Valerie J. Russell, Rustam Aminov, Harry J. Flint, Petra Louis

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Abstract

Bacterial butyryl-CoA CoA-transferase activity plays a key role in butyrate formation in the human colon, but the enzyme and corresponding gene responsible for this activity have not previously been identified. A novel CoA-transferase gene is described from the colonic bacterium Roseburia sp. A2-183, with similarity to acetyl-CoA hydrolase as well as 4-hydroxybutyrate CoA-transferase sequences. The gene product, overexpressed in an Escherichia coli lysate, showed activity with butyryl-CoA and to a lesser degree propionyl-CoA in the presence of acetate. Butyrate, propionate, isobutyrate and valerate competed with acetate as the co-substrate. Despite the sequence similarity to 4-hydroxybutyrate CoA-transferases, 4-hydroxybutyrate did not compete with acetate as the co-substrate. Thus the CoA-transferase preferentially uses butyryl-CoA as substrate. Similar genes were identified in other butyrate-producing human gut bacteria from clostridial clusters IV and XIVa, while other candidate CoA-transferases for butyrate formation could not be detected in Roseburia sp. A2-183. This suggests strongly that the newly identified group of CoA-transferases described here plays a key role in butyrate formation in the human colon.
Original languageEnglish
JournalMicrobiology
Volume152
Issue numberPart 1
Pages (from-to)179-185
Number of pages7
ISSN1350-0872
DOIs
Publication statusPublished - 2006
Externally publishedYes

Keywords

  • Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteroidaceae [06901] Roseburia genus strain-A2-183
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Escherichia coli species expression system
  • Roseburia CoA-transferase gene [Bacteroidaceae]
  • 4-hydroxybutyrate CoA-transferase
  • acetate 71-50-1
  • acetyl-CoA hydrolase 9027-54-7 EC 3.1.2.1
  • butyrate 461-55-2
  • butyryl-CoA 2140-48-9
  • CoA-transferase
  • isobutyrate 5711-69-3
  • propionate 72-03-7
  • propionyl-CoA 317-66-8
  • short-chain fatty acid
  • valerate 10023-74-2
  • 02502, Cytology - General
  • 03502, Genetics - General
  • 10066, Biochemistry studies - Lipids
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 30500, Morphology and cytology of bacteria
  • 31000, Physiology and biochemistry of bacteria
  • 31500, Genetics of bacteria and viruses
  • Biochemistry and Molecular Biophysics
  • Cell Biology
  • Enzymology
  • Molecular Genetics

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