A novel biomarker of MMP-cleaved prolargin is elevated in patients with psoriatic arthritis

Dovile Sinkeviciute*, Solveig Skovlund Groen, Shu Sun, Tina Manon-Jensen, Anders Aspberg, Patrik Önnerfjord, Anne Christine Bay-Jensen, Salome Kristensen, Signe Holm Nielsen

*Corresponding author for this work

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Abstract

Psoriatic arthritis (PsA) is a chronic musculoskeletal inflammatory disease found in up to 30% of psoriasis patients. Prolargin—an extracellular matrix (ECM) protein present in cartilage and tendon—has been previously shown elevated in serum of patients with psoriasis. ECM protein fragments can reflect tissue turnover and pathological changes; thus, this study aimed to develop, validate and characterize a novel biomarker PROM targeting a matrix metalloproteinase (MMP)-cleaved prolargin neo-epitope, and to evaluate it as a biomarker for PsA. A competitive ELISA was developed with a monoclonal mouse antibody; dilution- and spiking-recovery, inter- and intra-variation, and accuracy were evaluated. Serum levels were evaluated in 55 healthy individuals and 111 patients diagnosed with PsA by the CASPAR criteria. Results indicated that the PROM assay was specific for the neo-epitope. Inter- and intra- assay variations were 11% and 4%, respectively. PROM was elevated (p = 0.0003) in patients with PsA (median: 0.24, IQR: 0.19–0.31) compared to healthy controls (0.18; 0.14–0.23) at baseline. AUROC for separation of healthy controls from PsA patients was 0.674 (95% CI 0.597–0.744, P <0.001). In conclusion, MMP-cleaved prolargin can be quantified in serum by the PROM assay and has the potential to separate patients with PsA from healthy controls.
Original languageEnglish
Article number13541
JournalScientific Reports
Volume10
Issue number1
Number of pages10
ISSN2045-2322
DOIs
Publication statusPublished - 2020

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