A Novel Bio-Purification Process Employing an Engineered E. coli Strain for Downstream Processing of Lactic Acid Solutions from the Fermentation of Agro-Industrial by-Products

Alexandra Nastouli, Alexandra Moschona, Panagiotis A. Bizirtsakis, Joseph Sweeney, Irini Angelidaki, Michael Harasek, Anastasios J. Karabelas, Sotiris I. Patsios*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

9 Downloads (Pure)

Abstract

This study aims to integrate a novel bio-purification process employing an engineered E. coli strain in the downstream processing of lactic acid (LA) fermentation broths from low-cost renewable biological feedstocks. Fermentation broth of candy waste and digestate mixture was used as a real biological feedstock. An engineered E. coli strain that selectively catabolize impurities without catabolizing LA was initially adapted on the biological feedstock, followed by shake flask experiments to prove the bio-purification concept. Scale-up and validation in a bench-scale bioreactor followed, before developing a semi-continuous membrane bioreactor (MBR) bio-purification process. The MBR bio-purification was assessed with biological feedstocks which simulated ultrafiltration or nanofiltration permeates. Incomplete removal of impurities and increased fouling was observed in the case of the ultrafiltration permeate. Contrarily, the nanofiltration permeate was successfully treated with MBR bio-purification, since low membrane fouling, 100% maltose and acetic acid removal, and no LA catabolism was achieved. MBR bio-purification as a post-treatment step in the downstream processing of LA was demonstrated as a promising technology for increasing the purity of LA solutions.

Original languageEnglish
Article number412
JournalBioengineering
Volume11
Issue number5
Number of pages21
ISSN2306-5354
DOIs
Publication statusPublished - 2024

Keywords

  • Bio-purification
  • E. coli
  • Fermentation
  • Lactic acid
  • Membrane bioreactor
  • Nanofiltration
  • Selective separation
  • Semi-continuous operation
  • Ultrafiltration

Fingerprint

Dive into the research topics of 'A Novel Bio-Purification Process Employing an Engineered E. coli Strain for Downstream Processing of Lactic Acid Solutions from the Fermentation of Agro-Industrial by-Products'. Together they form a unique fingerprint.

Cite this