A novel archaeal species belonging to Methanoculleus genus identified via de-novo assembly and metagenomic binning process in biogas reactors

Panagiotis Kougias, Stefano Campanaro, Laura Treu, Xinyu Zhu, Irini Angelidaki

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Recently, a first comprehensive catalogue of microbial genomes populating biogas reactors treating manure and agro-industrial residues was determined by sequencing samples collected from 22 biogas reactors including laboratory and full scale. Among the archaeal community, one of the most abundant methanogens belongs to Methanoculleus genus and for this reason it was provisionally named Methanoculleus sp. DTU006. Its full length 16S rRNA sequence is 97% similar to Methanoculleus marisnigri JR1 and to Methanoculleus palmolei DSM 4273. Despite the high similarity of the 16S gene sequence, Average Nucleotide Identity calculation (ANI) calculated on all protein encoding genes indicated that the two most similar species, Methanoculleus bourgensis MS2T and Methanoculleus sp. MAB1, are divergent enough to define Methanoculleus sp. DTU006 as new archaeal species. Its genome (2.15 Mbp) has an estimated completeness around 93%. Analysis of the metabolic pathways using KEGG confirmed that it is a hydrogenotrophic methanogen and therefore it is proposed the Candidatus status by naming it as "Candidatus Methanoculleus thermohydrogenotrophicum".
Original languageEnglish
JournalAnaerobe
Volume46
Pages (from-to)23-32
ISSN1075-9964
DOIs
Publication statusPublished - 2017

Keywords

  • Anaerobic digestion
  • Archaea
  • Biogas
  • Hydrogenotroph
  • Metagenomic binning
  • Methanogen

Cite this

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title = "A novel archaeal species belonging to Methanoculleus genus identified via de-novo assembly and metagenomic binning process in biogas reactors",
abstract = "Recently, a first comprehensive catalogue of microbial genomes populating biogas reactors treating manure and agro-industrial residues was determined by sequencing samples collected from 22 biogas reactors including laboratory and full scale. Among the archaeal community, one of the most abundant methanogens belongs to Methanoculleus genus and for this reason it was provisionally named Methanoculleus sp. DTU006. Its full length 16S rRNA sequence is 97{\%} similar to Methanoculleus marisnigri JR1 and to Methanoculleus palmolei DSM 4273. Despite the high similarity of the 16S gene sequence, Average Nucleotide Identity calculation (ANI) calculated on all protein encoding genes indicated that the two most similar species, Methanoculleus bourgensis MS2T and Methanoculleus sp. MAB1, are divergent enough to define Methanoculleus sp. DTU006 as new archaeal species. Its genome (2.15 Mbp) has an estimated completeness around 93{\%}. Analysis of the metabolic pathways using KEGG confirmed that it is a hydrogenotrophic methanogen and therefore it is proposed the Candidatus status by naming it as {"}Candidatus Methanoculleus thermohydrogenotrophicum{"}.",
keywords = "Anaerobic digestion, Archaea, Biogas, Hydrogenotroph, Metagenomic binning, Methanogen",
author = "Panagiotis Kougias and Stefano Campanaro and Laura Treu and Xinyu Zhu and Irini Angelidaki",
year = "2017",
doi = "10.1016/j.anaerobe.2017.02.009",
language = "English",
volume = "46",
pages = "23--32",
journal = "Anaerobe",
issn = "1075-9964",
publisher = "Academic Press",

}

A novel archaeal species belonging to Methanoculleus genus identified via de-novo assembly and metagenomic binning process in biogas reactors. / Kougias, Panagiotis; Campanaro, Stefano; Treu, Laura; Zhu, Xinyu; Angelidaki, Irini.

In: Anaerobe, Vol. 46, 2017, p. 23-32.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - A novel archaeal species belonging to Methanoculleus genus identified via de-novo assembly and metagenomic binning process in biogas reactors

AU - Kougias, Panagiotis

AU - Campanaro, Stefano

AU - Treu, Laura

AU - Zhu, Xinyu

AU - Angelidaki, Irini

PY - 2017

Y1 - 2017

N2 - Recently, a first comprehensive catalogue of microbial genomes populating biogas reactors treating manure and agro-industrial residues was determined by sequencing samples collected from 22 biogas reactors including laboratory and full scale. Among the archaeal community, one of the most abundant methanogens belongs to Methanoculleus genus and for this reason it was provisionally named Methanoculleus sp. DTU006. Its full length 16S rRNA sequence is 97% similar to Methanoculleus marisnigri JR1 and to Methanoculleus palmolei DSM 4273. Despite the high similarity of the 16S gene sequence, Average Nucleotide Identity calculation (ANI) calculated on all protein encoding genes indicated that the two most similar species, Methanoculleus bourgensis MS2T and Methanoculleus sp. MAB1, are divergent enough to define Methanoculleus sp. DTU006 as new archaeal species. Its genome (2.15 Mbp) has an estimated completeness around 93%. Analysis of the metabolic pathways using KEGG confirmed that it is a hydrogenotrophic methanogen and therefore it is proposed the Candidatus status by naming it as "Candidatus Methanoculleus thermohydrogenotrophicum".

AB - Recently, a first comprehensive catalogue of microbial genomes populating biogas reactors treating manure and agro-industrial residues was determined by sequencing samples collected from 22 biogas reactors including laboratory and full scale. Among the archaeal community, one of the most abundant methanogens belongs to Methanoculleus genus and for this reason it was provisionally named Methanoculleus sp. DTU006. Its full length 16S rRNA sequence is 97% similar to Methanoculleus marisnigri JR1 and to Methanoculleus palmolei DSM 4273. Despite the high similarity of the 16S gene sequence, Average Nucleotide Identity calculation (ANI) calculated on all protein encoding genes indicated that the two most similar species, Methanoculleus bourgensis MS2T and Methanoculleus sp. MAB1, are divergent enough to define Methanoculleus sp. DTU006 as new archaeal species. Its genome (2.15 Mbp) has an estimated completeness around 93%. Analysis of the metabolic pathways using KEGG confirmed that it is a hydrogenotrophic methanogen and therefore it is proposed the Candidatus status by naming it as "Candidatus Methanoculleus thermohydrogenotrophicum".

KW - Anaerobic digestion

KW - Archaea

KW - Biogas

KW - Hydrogenotroph

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