A non-Gaussian distribution quantifies distances measured with fluorescence localization techniques

L.S. Churchman, H. Flyvbjerg, J.A. Spudich

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    When single-molecule fluorescence localization techniques are pushed to their lower limits in attempts to measure ever-shorter distances, measurement errors become important to understand. Here we describe the non-Gaussian distribution of measured distances that is the key to proper interpretation of distance measurements. We test it on single-molecule high-resolution colocalization data for a known distance, 10 nm, and find that it gives the correct result, whereas interpretation of the same data with a Gaussian distribution gives a result that is systematically too large.
    Original languageEnglish
    JournalBiophysical Journal
    Volume90
    Issue number2
    Pages (from-to)668-671
    ISSN0006-3495
    DOIs
    Publication statusPublished - 2006

    Fingerprint Dive into the research topics of 'A non-Gaussian distribution quantifies distances measured with fluorescence localization techniques'. Together they form a unique fingerprint.

    Cite this