Abstract
When single-molecule fluorescence localization techniques are pushed to their lower limits in attempts to measure ever-shorter distances, measurement errors become important to understand. Here we describe the non-Gaussian distribution of measured distances that is the key to proper interpretation of distance measurements. We test it on single-molecule high-resolution colocalization data for a known distance, 10 nm, and find that it gives the correct result, whereas interpretation of the same data with a Gaussian distribution gives a result that is systematically too large.
Original language | English |
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Journal | Biophysical Journal |
Volume | 90 |
Issue number | 2 |
Pages (from-to) | 668-671 |
ISSN | 0006-3495 |
DOIs | |
Publication status | Published - 2006 |