TY - JOUR
T1 - A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA
AU - Mohammadniaei, Mohsen
AU - Zhang, Ming
AU - Ashley, Jon
AU - Christensen, Ulf Bech
AU - Friis-Hansen, Lennart Jan
AU - Gregersen, Rasmus
AU - Lisby, Jan Gorm
AU - Benfield, Thomas Lars
AU - Nielsen, Finn Erland
AU - Henning Rasmussen, Jens
AU - Pedersen, Ellen Bøtker
AU - Olinger, Anne Christine Rye
AU - Kolding, Lærke Tørring
AU - Naseri, Maryam
AU - Zheng, Tao
AU - Wang, Wentao
AU - Gorodkin, Jan
AU - Sun, Yi
N1 - Funding Information:
This work is financially supported by European Institute of Innovation & Technology (EIT) Health, Project no. 20876.
PY - 2021
Y1 - 2021
N2 - The current nucleic acid signal amplification methods for SARS-CoV-2 RNA detection heavily rely on the functions of biological enzymes which imposes stringent transportation and storage conditions, high cost and global supply shortages. Here, a non-enzymatic whole genome detection method based on a simple isothermal signal amplification approach is developed for rapid detection of SARS-CoV-2 RNA and potentially any types of nucleic acids regardless of their size. The assay, termed non-enzymatic isothermal strand displacement and amplification (NISDA), is able to quantify 10 RNA copies.µL−1. In 164 clinical oropharyngeal RNA samples, NISDA assay is 100 % specific, and it is 96.77% and 100% sensitive when setting up in the laboratory and hospital, respectively. The NISDA assay does not require RNA reverse-transcription step and is fast (<30 min), affordable, highly robust at room temperature (>1 month), isothermal (42 °C) and user-friendly, making it an excellent assay for broad-based testing.
AB - The current nucleic acid signal amplification methods for SARS-CoV-2 RNA detection heavily rely on the functions of biological enzymes which imposes stringent transportation and storage conditions, high cost and global supply shortages. Here, a non-enzymatic whole genome detection method based on a simple isothermal signal amplification approach is developed for rapid detection of SARS-CoV-2 RNA and potentially any types of nucleic acids regardless of their size. The assay, termed non-enzymatic isothermal strand displacement and amplification (NISDA), is able to quantify 10 RNA copies.µL−1. In 164 clinical oropharyngeal RNA samples, NISDA assay is 100 % specific, and it is 96.77% and 100% sensitive when setting up in the laboratory and hospital, respectively. The NISDA assay does not require RNA reverse-transcription step and is fast (<30 min), affordable, highly robust at room temperature (>1 month), isothermal (42 °C) and user-friendly, making it an excellent assay for broad-based testing.
U2 - 10.1038/s41467-021-25387-9
DO - 10.1038/s41467-021-25387-9
M3 - Journal article
C2 - 34429424
AN - SCOPUS:85113296116
SN - 2041-1723
VL - 12
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 5089
ER -