A next generation sequencing approach for targeted Varroa destructor (Acari Varroidae) mitochondrial DNA analysis based on honey derived environmental DNA

Research output: Contribution to journalJournal article – Annual report year: 2019Researchpeer-review

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A next generation sequencing approach for targeted Varroa destructor (Acari Varroidae) mitochondrial DNA analysis based on honey derived environmental DNA. / Utzeri, Valerio Joe; Schiavo, Giuseppina; Ribani, Anisa; Bertolini, Francesca; Bovo, Samuele; Fontanesi, Luca.

In: Journal of Invertebrate Pathology, Vol. 161, 2019, p. 47-53.

Research output: Contribution to journalJournal article – Annual report year: 2019Researchpeer-review

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@article{de01f1310066459d9391abfebeb861fa,
title = "A next generation sequencing approach for targeted Varroa destructor (Acari Varroidae) mitochondrial DNA analysis based on honey derived environmental DNA",
abstract = "Honey contains DNA from many different organisms that are part of hive micro-environmental niches and honey bee pathospheres. In this study, we recovered and sequenced mite mitochondrial DNA (mtDNA) from honey from different locations around the world (Europe, Asia, Africa, North and South America). DNA extracted from 17 honey samples was amplified with eight primer pairs targeting three mite mtDNA genes, obtaining 88 amplicons that were sequenced with an Ion Torrent sequencing platform. A bioinformatic pipeline compared produced reads with Varroa spp. mtDNA sequence entries available in GenBank and assigned them to different mitotypes. In all honey samples, the highest percentage of reads was attributed to the K1 lineage, including a few variants derived from it, in addition to J1 reads observed in the two South American samples and C1-1 reads obtained from the Chinese honey. This study opens new possibilities to analyse mite lineages and variants and monitor their geographical and temporal distribution, simplifying surveillance against this damaging honey bee parasite.",
keywords = "eDNA, Honey bee, Lineages, NGS, Parasite, Variants",
author = "Utzeri, {Valerio Joe} and Giuseppina Schiavo and Anisa Ribani and Francesca Bertolini and Samuele Bovo and Luca Fontanesi",
year = "2019",
doi = "10.1016/j.jip.2019.01.005",
language = "English",
volume = "161",
pages = "47--53",
journal = "Journal of Invertebrate Pathology",
issn = "0022-2011",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - A next generation sequencing approach for targeted Varroa destructor (Acari Varroidae) mitochondrial DNA analysis based on honey derived environmental DNA

AU - Utzeri, Valerio Joe

AU - Schiavo, Giuseppina

AU - Ribani, Anisa

AU - Bertolini, Francesca

AU - Bovo, Samuele

AU - Fontanesi, Luca

PY - 2019

Y1 - 2019

N2 - Honey contains DNA from many different organisms that are part of hive micro-environmental niches and honey bee pathospheres. In this study, we recovered and sequenced mite mitochondrial DNA (mtDNA) from honey from different locations around the world (Europe, Asia, Africa, North and South America). DNA extracted from 17 honey samples was amplified with eight primer pairs targeting three mite mtDNA genes, obtaining 88 amplicons that were sequenced with an Ion Torrent sequencing platform. A bioinformatic pipeline compared produced reads with Varroa spp. mtDNA sequence entries available in GenBank and assigned them to different mitotypes. In all honey samples, the highest percentage of reads was attributed to the K1 lineage, including a few variants derived from it, in addition to J1 reads observed in the two South American samples and C1-1 reads obtained from the Chinese honey. This study opens new possibilities to analyse mite lineages and variants and monitor their geographical and temporal distribution, simplifying surveillance against this damaging honey bee parasite.

AB - Honey contains DNA from many different organisms that are part of hive micro-environmental niches and honey bee pathospheres. In this study, we recovered and sequenced mite mitochondrial DNA (mtDNA) from honey from different locations around the world (Europe, Asia, Africa, North and South America). DNA extracted from 17 honey samples was amplified with eight primer pairs targeting three mite mtDNA genes, obtaining 88 amplicons that were sequenced with an Ion Torrent sequencing platform. A bioinformatic pipeline compared produced reads with Varroa spp. mtDNA sequence entries available in GenBank and assigned them to different mitotypes. In all honey samples, the highest percentage of reads was attributed to the K1 lineage, including a few variants derived from it, in addition to J1 reads observed in the two South American samples and C1-1 reads obtained from the Chinese honey. This study opens new possibilities to analyse mite lineages and variants and monitor their geographical and temporal distribution, simplifying surveillance against this damaging honey bee parasite.

KW - eDNA

KW - Honey bee

KW - Lineages

KW - NGS

KW - Parasite

KW - Variants

U2 - 10.1016/j.jip.2019.01.005

DO - 10.1016/j.jip.2019.01.005

M3 - Journal article

VL - 161

SP - 47

EP - 53

JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

SN - 0022-2011

ER -