A new method to measure mechanics and dynamic assembly of branched actin networks

Pierre Bauër, Joe Tavacoli, Thomas Pujol, Jessica Planade, Julien Heuvingh, Olivia Du Roure

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Abstract

We measured mechanical properties and dynamic assembly of actin networks with a new method based on magnetic microscopic cylinders. Dense actin networks are grown from the cylinders’ surfaces using the biochemical Arp2/3-machinery at play in the lamellipodium extension and other force-generating processes in the cell. Under a homogenous magnetic field the magnetic cylinders self-assemble into chains in which forces are attractive and depend on the intensity of the magnetic field. We show that these forces, from piconewtons to nanonewtons, are large enough to slow down the assembly of dense actin networks and controlled enough to access to their non linear mechanical responses. Deformations are measured with nanometer-resolution, well below the optical resolution. Self-assembly of the magnetic particles into chains simplifies experiments and allows for parallel measurements. The combination of accuracy and good throughput of measurements results in a method with high potential for cell and cytoskeleton mechanics. Using this method, we observed in particular a strong non linear mechanical behavior of dense branched actin networks at low forces that has not been reported previously.
Original languageEnglish
Article number15688
JournalScientific Reports
Volume7
Number of pages11
ISSN2045-2322
DOIs
Publication statusPublished - 2017

Cite this

Bauër, P., Tavacoli, J., Pujol, T., Planade, J., Heuvingh, J., & Du Roure, O. (2017). A new method to measure mechanics and dynamic assembly of branched actin networks. Scientific Reports, 7, [15688]. https://doi.org/10.1038/s41598-017-15638-5
Bauër, Pierre ; Tavacoli, Joe ; Pujol, Thomas ; Planade, Jessica ; Heuvingh, Julien ; Du Roure, Olivia. / A new method to measure mechanics and dynamic assembly of branched actin networks. In: Scientific Reports. 2017 ; Vol. 7.
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abstract = "We measured mechanical properties and dynamic assembly of actin networks with a new method based on magnetic microscopic cylinders. Dense actin networks are grown from the cylinders’ surfaces using the biochemical Arp2/3-machinery at play in the lamellipodium extension and other force-generating processes in the cell. Under a homogenous magnetic field the magnetic cylinders self-assemble into chains in which forces are attractive and depend on the intensity of the magnetic field. We show that these forces, from piconewtons to nanonewtons, are large enough to slow down the assembly of dense actin networks and controlled enough to access to their non linear mechanical responses. Deformations are measured with nanometer-resolution, well below the optical resolution. Self-assembly of the magnetic particles into chains simplifies experiments and allows for parallel measurements. The combination of accuracy and good throughput of measurements results in a method with high potential for cell and cytoskeleton mechanics. Using this method, we observed in particular a strong non linear mechanical behavior of dense branched actin networks at low forces that has not been reported previously.",
author = "Pierre Bau{\"e}r and Joe Tavacoli and Thomas Pujol and Jessica Planade and Julien Heuvingh and {Du Roure}, Olivia",
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Bauër, P, Tavacoli, J, Pujol, T, Planade, J, Heuvingh, J & Du Roure, O 2017, 'A new method to measure mechanics and dynamic assembly of branched actin networks', Scientific Reports, vol. 7, 15688. https://doi.org/10.1038/s41598-017-15638-5

A new method to measure mechanics and dynamic assembly of branched actin networks. / Bauër, Pierre ; Tavacoli, Joe; Pujol, Thomas ; Planade, Jessica ; Heuvingh, Julien; Du Roure, Olivia.

In: Scientific Reports, Vol. 7, 15688, 2017.

Research output: Contribution to journalJournal articleResearchpeer-review

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AU - Tavacoli, Joe

AU - Pujol, Thomas

AU - Planade, Jessica

AU - Heuvingh, Julien

AU - Du Roure, Olivia

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AB - We measured mechanical properties and dynamic assembly of actin networks with a new method based on magnetic microscopic cylinders. Dense actin networks are grown from the cylinders’ surfaces using the biochemical Arp2/3-machinery at play in the lamellipodium extension and other force-generating processes in the cell. Under a homogenous magnetic field the magnetic cylinders self-assemble into chains in which forces are attractive and depend on the intensity of the magnetic field. We show that these forces, from piconewtons to nanonewtons, are large enough to slow down the assembly of dense actin networks and controlled enough to access to their non linear mechanical responses. Deformations are measured with nanometer-resolution, well below the optical resolution. Self-assembly of the magnetic particles into chains simplifies experiments and allows for parallel measurements. The combination of accuracy and good throughput of measurements results in a method with high potential for cell and cytoskeleton mechanics. Using this method, we observed in particular a strong non linear mechanical behavior of dense branched actin networks at low forces that has not been reported previously.

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