Abstract
Herein, we present the use of a single gold nanorod sensor for detection of diseases on an antibodyfunctionalized
surface, based on antibody–antigen interaction and the localized surface plasmon
resonance (LSPR) lmax shifts of the resonant Rayleigh light scattering spectra. By replacing the
cetyltrimethylammonium bromide (CTAB), a tightly packed self-assembled monolayer of HS
(CH2)11(OCH2CH2)6OCH2COOH(OEG6) has been successfully formed on the gold nanorod surface
prior to the LSPR sensing, leading to the successful fabrication of individual gold nanorod
immunosensors. Using prostate specific antigen (PSA) as a protein biomarker, the lowest concentration
experimentally detected was as low as 111 aM, corresponding to a 2.79 nm LSPR lmax shift. These
results indicate that the detection platform is very sensitive and outperforms detection limits of
commercial tests for PSA so far. Correlatively, its detection limit can be equally compared to the assays
based on DNA biobarcodes. This study shows that a gold nanorod has been used as a single
nanobiosensor to detect antigens for the first time; and the detection method based on the resonant
Rayleigh scattering spectrum of individual gold nanorods enables a simple, label-free detection with
ultrahigh sensitivity.
Original language | English |
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Journal | Lab on a Chip |
Volume | 11 |
Pages (from-to) | 2591-2597 |
ISSN | 1473-0197 |
DOIs | |
Publication status | Published - 2011 |