A new member of the LIR family from perennial ryegrass is cold-responsive, and promotes vegetative growth in ¤Arabidopsis¤

S. Ciannamea; Christian Sig Jensen; Henrik Agerskov; Klaus Petersen; I. Lenk; Thomas Didion; R.G.H. Immink; G.C. Angenent; Klaus K. Nielsen

doi:10.1016/j.plantsci.2006.08.011

A new member of the LIR family from perennial ryegrass is cold-responsive, and promotes vegetative growth in ¤Arabidopsis¤

S. Ciannamea, Christian Sig Jensen, Henrik Agerskov, Klaus Petersen, I. Lenk, Thomas Didion, R.G.H. Immink, G.C. Angenent, Klaus K. Nielsen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A cold-regulated gene Lolium perenne LIR1 (LpLIR1) was isolated from perennial ryegrass using a subtractive approach. The gene has strong homology to the Light Induced Rice1 (LIR1) gene and is regulated at the transcriptional level by cold, and by a diurnal rhythm. Expression of LpLIR1 in perennial ryegrass was upregulated by vernalization but did not follow a standard vernalization-responsive expression pattern. LpLIR1 expression was restricted to vegetative tissues and absent in apices during floral induction and in flowers. LpLIR1 mRNA levels displayed diurnal fluctuations, which peaked before dusk and declined during the night. Heterologous expression of LpLIR1 in Arabidopsis led to a significant increase in leaf formation under short days (SD) conditions but only when plants had received a preceding vernalization treatment. Furthermore, dissection of plant development under SD revealed a minor but significant delay of flowering in the transgenic lines compared to wildtype plants. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

Original language	English
Journal	Plant Science
Volume	172
Issue number	2
Pages (from-to)	221-227
ISSN	0168-9452
DOIs	https://doi.org/10.1016/j.plantsci.2006.08.011
Publication status	Published - 2007

Access to Document

10.1016/j.plantsci.2006.08.011

OpenUrl availability

Full text

Cite this

@article{b50f844a38c94276ace59429a248b5c3,

title = "A new member of the LIR family from perennial ryegrass is cold-responsive, and promotes vegetative growth in ¤Arabidopsis¤",

abstract = "A cold-regulated gene Lolium perenne LIR1 (LpLIR1) was isolated from perennial ryegrass using a subtractive approach. The gene has strong homology to the Light Induced Rice1 (LIR1) gene and is regulated at the transcriptional level by cold, and by a diurnal rhythm. Expression of LpLIR1 in perennial ryegrass was upregulated by vernalization but did not follow a standard vernalization-responsive expression pattern. LpLIR1 expression was restricted to vegetative tissues and absent in apices during floral induction and in flowers. LpLIR1 mRNA levels displayed diurnal fluctuations, which peaked before dusk and declined during the night. Heterologous expression of LpLIR1 in Arabidopsis led to a significant increase in leaf formation under short days (SD) conditions but only when plants had received a preceding vernalization treatment. Furthermore, dissection of plant development under SD revealed a minor but significant delay of flowering in the transgenic lines compared to wildtype plants. (c) 2006 Elsevier Ireland Ltd. All rights reserved.",

keywords = "Medikomaterialer, Nanobioteknologi",

author = "S. Ciannamea and Jensen, {Christian Sig} and Henrik Agerskov and Klaus Petersen and I. Lenk and Thomas Didion and R.G.H. Immink and G.C. Angenent and Nielsen, {Klaus K.}",

year = "2007",

doi = "10.1016/j.plantsci.2006.08.011",

language = "English",

volume = "172",

pages = "221--227",

journal = "Plant Science",

issn = "0168-9452",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - A new member of the LIR family from perennial ryegrass is cold-responsive, and promotes vegetative growth in ¤Arabidopsis¤

AU - Ciannamea, S.

AU - Jensen, Christian Sig

AU - Agerskov, Henrik

AU - Petersen, Klaus

AU - Lenk, I.

AU - Didion, Thomas

AU - Immink, R.G.H.

AU - Angenent, G.C.

AU - Nielsen, Klaus K.

PY - 2007

Y1 - 2007

N2 - A cold-regulated gene Lolium perenne LIR1 (LpLIR1) was isolated from perennial ryegrass using a subtractive approach. The gene has strong homology to the Light Induced Rice1 (LIR1) gene and is regulated at the transcriptional level by cold, and by a diurnal rhythm. Expression of LpLIR1 in perennial ryegrass was upregulated by vernalization but did not follow a standard vernalization-responsive expression pattern. LpLIR1 expression was restricted to vegetative tissues and absent in apices during floral induction and in flowers. LpLIR1 mRNA levels displayed diurnal fluctuations, which peaked before dusk and declined during the night. Heterologous expression of LpLIR1 in Arabidopsis led to a significant increase in leaf formation under short days (SD) conditions but only when plants had received a preceding vernalization treatment. Furthermore, dissection of plant development under SD revealed a minor but significant delay of flowering in the transgenic lines compared to wildtype plants. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

AB - A cold-regulated gene Lolium perenne LIR1 (LpLIR1) was isolated from perennial ryegrass using a subtractive approach. The gene has strong homology to the Light Induced Rice1 (LIR1) gene and is regulated at the transcriptional level by cold, and by a diurnal rhythm. Expression of LpLIR1 in perennial ryegrass was upregulated by vernalization but did not follow a standard vernalization-responsive expression pattern. LpLIR1 expression was restricted to vegetative tissues and absent in apices during floral induction and in flowers. LpLIR1 mRNA levels displayed diurnal fluctuations, which peaked before dusk and declined during the night. Heterologous expression of LpLIR1 in Arabidopsis led to a significant increase in leaf formation under short days (SD) conditions but only when plants had received a preceding vernalization treatment. Furthermore, dissection of plant development under SD revealed a minor but significant delay of flowering in the transgenic lines compared to wildtype plants. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

KW - Medikomaterialer

KW - Nanobioteknologi

U2 - 10.1016/j.plantsci.2006.08.011

DO - 10.1016/j.plantsci.2006.08.011

M3 - Journal article

SN - 0168-9452

VL - 172

SP - 221

EP - 227

JO - Plant Science

JF - Plant Science

IS - 2

ER -

A new member of the LIR family from perennial ryegrass is cold-responsive, and promotes vegetative growth in ¤Arabidopsis¤

Abstract

Access to Document

OpenUrl availability

Fingerprint

Cite this