A new high-throughput LC-MS method for the analysis of complex fructan mixtures.

Joran Verspreet, Anders Holmgaard Hansen, Emmie Dornez, Christophe M. Courtin, Scott James Harrison

Research output: Contribution to journalJournal articleResearchpeer-review


In this paper, a new liquid chromatography-mass spectrometry (LC-MS) method for the analysis of complex fructan mixtures is presented. In this method, columns with a trifunctional C18 alkyl stationary phase (T3) were used and their performance compared with that of a porous graphitized carbon (PGC) column. The separation of fructan isomers with the T3 phase improved clearly in comparison with the PGC phase, and retention times were lower and more stable. When the T3-based method was applied on a wheat grain extract, multiple fructan isomers could be discerned, even for fructans with a degree of polymerization of 10. This indicates that wheat grain fructans do not, or not only, have a simple linear structure. The presented method paves the way for elucidation of fructan structures in complex mixtures that contain many structural isomers.
Original languageEnglish
JournalAnalytical and Bioanalytical Chemistry
Issue number19
Pages (from-to)4785-4788
Number of pages4
Publication statusPublished - 2014


  • Fructan
  • Trifunctional C18
  • Porous graphitized carbon
  • Liquid chromatography-mass spectrometry
  • Wheat grains


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