A New Extant Respirometric Assay to Estimate Intrinsic Growth Parameters Applied to Study Plasmid Metabolic Burden

Jose Miguel Seoane, Gürkan Sin, Laurent Lardon, Krist Gernaey, Barth F. Smets

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Start-Lip phenomena in microbial biokinetic assays are not captured by the most commonly used growth-related equations. In this Study we propose a new respirometric experimental design to estimate intrinsic growth parameters that allow us to avoid these limitations without data omission, separate mathematical treatment, or wake-up pulses prior to the analysis.. Identifiability and sensitivity analysis were performed to confirm the robustness of the new approach for obtaining unique and accurate estimates of growth kinetic parameters. The new experimental design was applied to establish the. metabolic burden caused by the carriage of a pWW0 TOL plasmid in the model organism Pseudomonas putida KT2440; The metabolic,burden associated was manifested as a reduction in the yield and the specific growth rate of the host, with both plasmid maintenance and the over-expression of recombinant proteins from the plasmid contributing equally to the overall effect. Biotechnol. Bioeng. 2010;105: 141-149. (C) 2009 Wiley Periodicals, Inc.
Original languageEnglish
JournalBiotechnology and Bioengineering (Print)
Volume105
Issue number1
Pages (from-to)141-149
ISSN0006-3592
DOIs
Publication statusPublished - 2010

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